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Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis†.

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cam.depositDate2022-01-12
cam.issuedOnline2022-01-17
cam.oa.sapoa_rrs_na
cam.orpheus.successTue Feb 01 19:02:43 GMT 2022 - Embargo updated
dc.contributor.authorWooding, FBP
dc.contributor.authorForhead, AJ
dc.contributor.authorWilsher, S
dc.contributor.authorAllen, WR
dc.contributor.authorRoberts, RM
dc.contributor.authorGreen, JA
dc.contributor.authorBeckers, JF
dc.contributor.authorSousa, N Melo
dc.contributor.authorCharpigny, G
dc.contributor.orcidWooding, Peter [0000-0003-2471-7586]
dc.contributor.orcidForhead, Alison [0000-0003-2125-4811]
dc.date.accessioned2022-01-13T00:31:44Z
dc.date.available2022-01-13T00:31:44Z
dc.date.issued2022-04-26
dc.date.updated2022-01-12T09:32:58Z
dc.description.abstractMature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 μm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system.
dc.description.sponsorshipNONE
dc.identifier.doi10.17863/CAM.80123
dc.identifier.eissn1529-7268
dc.identifier.issn0006-3363
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332678
dc.publisherOxford University Press (OUP)
dc.publisher.departmentDepartment of Physiology, Development And Neuroscience
dc.publisher.urlhttp://dx.doi.org/10.1093/biolre/ioab247
dc.rightsAll Rights Reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.subjectGiraffe granule protein asymmetry
dc.subjectRuminant placenta
dc.subjectTrophoblast binucleate cell
dc.subjectAnimals
dc.subjectFemale
dc.subjectGiraffes
dc.subjectLectins
dc.subjectPlacenta
dc.subjectPregnancy
dc.subjectProlactin
dc.subjectRuminants
dc.subjectTrophoblasts
dc.titleAsymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis†.
dc.typeArticle
dcterms.dateAccepted2022-01-15
prism.publicationNameBiol Reprod
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
pubs.licence-identifierapollo-deposit-licence-2-1
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1093/biolre/ioab247

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