Supplementary and raw data supporting the manuscript "Chronic inorganic nitrate supplementation does not improve metabolic health and worsens disease progression in mice with diet-induced obesity"

A study investigating the changes in whole-body, skeletal muscle, cardiac and hepatic metabolism associated with diet-induced obesity in mice recieving inorganic nitrate in their drinking water until 4-, 8- or 12-months of age.

XLSX data file containing raw data from main figures/tables and supplementary figures. Data contains a variety of morphometric and metabolic parameters, collected by techniques including (but not limited to) high-resolution respitometry (HRR), liquid chromatrography-mass spectrometry (LCMS) metabolite profiling, RT-qPCR, metabolic cage analysis analysis and histology. Full details provided in the manuscript; all units included in the XLSX.

PDF file contains 5 supplementary figures and 2 supplementary tables accompanying the manuscript. Supp. Fig. 1: Summary of statistical workflow used in the manuscript. Supp. Fig. 2: Mitochondrial capacity of mouse skeletal muscle and histological images of gastrointestinal tracts. Supp. Fig. 3: Acyl-carnitine changes in mouse hearts, measured by LCMS and cardiac mitochondrial capacity measured by HRR. Supp. Fig. 4: Lipidomic profiling from mouse livers (measured by LCMS). Supp. Fig. 5: Acyl-carnitine changes in mouse livers, measured by LCMS and hepatic mitochondrial capacity measured by HRR. Supp. Table 1: Primers used for RT-qPCR experiments. Supp. Table 2: Histological scoring system for MASLD activity score.

For further information see main manuscript.

XLSX file can be opened in Microsoft Excel; PDF can be opened in any pdf reader.