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ACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis.

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cam.issuedOnline2014-04-15
dc.contributor.authorQin, Yuan
dc.contributor.authorZhao, Lihua
dc.contributor.authorSkaggs, Megan I
dc.contributor.authorAndreuzza, Sebastien
dc.contributor.authorTsukamoto, Tatsuya
dc.contributor.authorPanoli, Aneesh
dc.contributor.authorWallace, Kirsten N
dc.contributor.authorSmith, Steven
dc.contributor.authorSiddiqi, Imran
dc.contributor.authorYang, Zhenbiao
dc.contributor.authorYadegari, Ramin
dc.contributor.authorPalanivelu, Ravishankar
dc.contributor.orcidYadegari, Ramin [0000-0002-0975-2984]
dc.contributor.orcidPalanivelu, Ravishankar [0000-0003-0564-5564]
dc.date.accessioned2019-06-24T13:09:33Z
dc.date.available2019-06-24T13:09:33Z
dc.date.issued2014-04
dc.description.abstractIn flowering plants, meiocytes develop from subepidermal cells in anthers and ovules. The mechanisms that integrate gene-regulatory processes with meiotic programs during reproductive development remain poorly characterized. Here, we show that Arabidopsis thaliana plants deficient in ACTIN-RELATED PROTEIN6 (ARP6), a subunit of the SWR1 ATP-dependent chromatin-remodeling complex, exhibit defects in prophase I of female meiosis. We found that this meiotic defect is likely due to dysregulated expression of meiotic genes, particularly those involved in meiotic recombination, including DMC1 (DISRUPTED MEIOTIC cDNA1). Analysis of DMC1 expression in arp6 mutant plants indicated that ARP6 inhibits expression of DMC1 in the megasporocyte and surrounding nonsporogeneous ovule cells before meiosis. After cells enter meiosis, however, ARP6 activates DMC1 expression specifically in the megasporocyte even as it continues to inhibit DMC1 expression in the nonsporogenous ovule cells. We further show that deposition of the histone variant H2A.Z, mediated by the SWR1 chromatin-remodeling complex at the DMC1 gene body, requires ARP6. Therefore, ARP6 regulates female meiosis by determining the spatial and temporal patterns of gene expression required for proper meiosis during ovule development.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.40998
dc.identifier.eissn1532-298X
dc.identifier.issn1040-4651
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/293890
dc.languageeng
dc.language.isoeng
dc.publisherOxford University Press (OUP)
dc.publisher.urlhttp://dx.doi.org/10.1105/tpc.113.120576
dc.subject0604 Genetics
dc.subject0607 Plant Biology
dc.subjectBiomedical
dc.subjectBasic Science
dc.subjectGenetics
dc.subjectBiotechnology
dc.subject1.1 Normal biological development and functioning
dc.titleACTIN-RELATED PROTEIN6 Regulates Female Meiosis by Modulating Meiotic Gene Expression in Arabidopsis.
dc.typeArticle
prism.endingPage1628
prism.issueIdentifier4
prism.publicationDate2014
prism.publicationNamePlant Cell
prism.startingPage1612
prism.volume26
rioxxterms.licenseref.startdate2014-04-15
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1105/tpc.113.120576

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