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Surface electrodes record and label brain neurons in insects.

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cam.issuedOnline2017-09-13
dc.contributor.authorKostarakos, Konstantinos
dc.contributor.authorHedwig, Berthold
dc.contributor.orcidHedwig, Berthold [0000-0002-1132-0056]
dc.date.accessioned2018-11-21T00:31:24Z
dc.date.available2018-11-21T00:31:24Z
dc.date.issued2017-11-01
dc.description.abstractWe used suction electrodes to reliably record the activity of identified ascending auditory interneurons from the anterior surface of the brain in crickets. Electrodes were gently attached to the sheath covering the projection area of the ascending interneurons and the ringlike auditory neuropil in the protocerebrum. The specificity and selectivity of the recordings were determined by the precise electrode location, which could easily be changed without causing damage to the tissue. Different nonauditory fibers were recorded at other spots of the brain surface; stable recordings lasted for several hours. The same electrodes were used to deliver fluorescent tracers into the nervous system by means of electrophoresis. This allowed us to retrograde label the recorded auditory neurons and to reveal their cell body and dendritic structure in the first thoracic ganglion. By adjusting the amount of dye injected, we specifically stained the ringlike auditory neuropil in the brain, demonstrating the clusters of cell bodies contributing to it. Our data provide a proof that surface electrodes are a versatile tool to analyze neural processing in small brains of invertebrates.NEW & NOTEWORTHY We show that surface suction electrodes can be used to monitor the activity of auditory neurons in the cricket brain. They also allow delivering electrophoretically a fluorescent tracer to label the structure of the recorded neurons and the local neuropil to which the electrode was attached. This new extracellular recording and labeling technique is a versatile and useful method to explore neural processing in invertebrate sensory and motor systems.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.32896
dc.identifier.eissn1522-1598
dc.identifier.issn0022-3077
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285539
dc.languageeng
dc.language.isoeng
dc.publisherAmerican Physiological Society
dc.publisher.urlhttp://dx.doi.org/10.1152/jn.00490.2017
dc.subjectauditory neurons
dc.subjectbrain
dc.subjectelectrophoretic staining
dc.subjectsingle cell recordings
dc.subjectsuction electrodes
dc.subjectAcoustic Stimulation
dc.subjectAction Potentials
dc.subjectAnimals
dc.subjectAuditory Perception
dc.subjectBrain
dc.subjectElectrodes
dc.subjectFemale
dc.subjectFluorescent Dyes
dc.subjectGryllidae
dc.subjectNeuroanatomical Tract-Tracing Techniques
dc.subjectNeurons
dc.subjectRestraint, Physical
dc.subjectSignal Processing, Computer-Assisted
dc.titleSurface electrodes record and label brain neurons in insects.
dc.typeArticle
dcterms.dateAccepted2017-09-06
prism.endingPage2889
prism.issueIdentifier5
prism.publicationDate2017
prism.publicationNameJ Neurophysiol
prism.startingPage2884
prism.volume118
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/J01835X/1)
rioxxterms.licenseref.startdate2017-11
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1152/jn.00490.2017

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