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Radiolabelled leucocytes in human pulmonary disease.

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cam.issuedOnline2018-07-24
dc.contributor.authorFarahi, Neda
dc.contributor.authorLoutsios, Chrystalla
dc.contributor.authorTregay, Nicola
dc.contributor.authorSummers, Charlotte
dc.contributor.authorLok, Laurence SC
dc.contributor.authorRuparelia, Prina
dc.contributor.authorSolanki, Chandra K
dc.contributor.authorGillett, Daniel
dc.contributor.authorChilvers, Edwin R
dc.contributor.authorPeters, A Michael
dc.contributor.orcidSummers, Charlotte [0000-0002-7269-2873]
dc.contributor.orcidLok, Laurence [0000-0002-9364-4213]
dc.contributor.orcidChilvers, Edwin [0000-0002-4230-9677]
dc.date.accessioned2018-09-27T14:11:18Z
dc.date.available2018-09-27T14:11:18Z
dc.date.issued2018-09-01
dc.description.abstractINTRODUCTION: Radionuclides for leucocyte kinetic studies have progressed from non-gamma emitting cell-labelling radionuclides through gamma emitting nuclides that allow imaging of leucocyte kinetics, to the next goal of positron emission tomography (PET). SOURCES OF DATA: Mostly the authors' own studies, following on from studies of the early pioneers. AREAS OF CONTROVERSY: From early imaging studies, it appeared that the majority of the marginated granulocyte pool was located in the lungs. However, later work disputed this by demonstrating the exquisite sensitivity of granulocytes to ex vivo isolation and labelling, and that excessive lung activity is artefactual. AREAS OF AGREEMENT: Following refinement of labelling techniques, it was shown that the majority of marginated granulocytes are located in the spleen and bone marrow. The majority of leucocytes have a pulmonary vascular transit time only a few seconds longer than erythrocytes. The minority showing slow transit, ~5% in healthy persons, is increased in systemic inflammatory disorders that cause neutrophil priming and loss of deformability. Using a range of imaging techniques, including gamma camera imaging, whole-body counting and single photon-emission computerized tomography, labelled granulocytes were subsequently used to image pulmonary trafficking in lobar pneumonia, bronchiectasis, chronic obstructive pulmonary disease and adult respiratory distress syndrome. GROWING POINTS: More recently, eosinophils have been separated in pure form using magnetic bead technology for the study of eosinophil trafficking in asthma. AREAS TIMELY FOR DEVELOPING RESEARCH: These include advancement of eosinophil imaging, development of monocyte labelling, development of cell labelling with PET tracers and the tracking of lymphocytes.
dc.description.sponsorshipAsthma UK, NIHR Cambridge Biomedical Research Centre, Royal Papworth Hospital, AstraZeneca, GlaxoSmithKline
dc.format.mediumPrint
dc.identifier.doi10.17863/CAM.30182
dc.identifier.eissn1471-8391
dc.identifier.issn0007-1420
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/282818
dc.languageeng
dc.language.isoeng
dc.publisherOxford University Press (OUP)
dc.publisher.urlhttp://dx.doi.org/10.1093/bmb/ldy022
dc.subjectGranulocytes
dc.subjectHumans
dc.subjectIsotope Labeling
dc.subjectLeukocytes
dc.subjectLung Diseases
dc.subjectPositron-Emission Tomography
dc.subjectRadioisotopes
dc.titleRadiolabelled leucocytes in human pulmonary disease.
dc.typeArticle
dcterms.dateAccepted2018-07-20
prism.endingPage82
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameBr Med Bull
prism.startingPage69
prism.volume127
pubs.funder-project-idMedical Research Council (MR/J00345X/1)
pubs.funder-project-idAsthma UK (05/066)
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (unknown)
pubs.funder-project-idAddenbrooke's Charitable Trust (ACT) (mINUTE 14/16 C (IV))
pubs.funder-project-idCambridge University Hospitals NHS Foundation Trust (CUH) (146281)
rioxxterms.licenseref.startdate2018-09
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionAM
rioxxterms.versionofrecord10.1093/bmb/ldy022

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