Research data in support of: "DNA-origami line-actants control domain organisation and fission in synthetic membranes"
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Repository DOI
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Authors
Mognetti, Bortolo
Cicuta, Pietro
Di Michele, Lorenzo
Description
- Confocal, epifluorescence, and brightfield micrographs of phase-separated Giant Unilamellar Vesicles functionalised with DNA-origami nanostructures.
- Atomic Force Microscopy micrograph of DNA origami tiles
- Agarose gel electrophoresis micrographs of DNA origami tiles
- Coarse-grained Monte Carlo simulation trajectories in image stacks and quantified observables (line-length, fraction of tiles at line, and fraction of occupied line) in text files.
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Software / Usage instructions
Image analysis and processing was carried out in MATLAB. FIJI was used to generate the 3D views from typical confocal z-stacks (with the Volume Viewer plugin)
Keywords
artificial cells, DNA nanotechnology, lipid domains, lipid phase separation, synthetic membranes
Publisher
Sponsorship
EPSRC (1949809)
Royal Society (UF160152)
Royal Society (RF\ERE\210029)
Royal Society (UF160152)
Royal Society (RF\ERE\210029)
R.R.S also acknowledges funding support from the Mexican National Council for Science and Technology (CONACYT, Grant No. 472427) and the Cambridge Trust.R.R.S. and L.D.M. also acknowledge funding from the Royal Society Research Fellows Enhanced Research Expenses (RF/ERE/210029) and from the European Research Council (ERC) under the Horizon 2020 Research and Innovation Programme (ERC-STG No 851667 NANOCELL). The authors acknowledge computational resources provided by the Consortium des Equipements de Calcul Intensif (CECI), funded by the Fonds de la Recherche Scientifique de Belgique (F.R.S.-FNRS) under Grant No. 2.5020.11 and by the Walloon Region.