Research data supporting "Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella"
These data have been generated as part of research projects aiming to characterise better the interplay between innate and adaptive immune systems. in particular, these data show that the Nod-like receptor (NLR) of the immune system NLRC4, which recognises microbial flagellin, downregulate the CD4+ T cell-mediated responses against the important zoonotic pathogen Salmonella Typhimurium in mice. Most importantly, we show that mice immunised with Salmonella that largely evades NLRC4 activation are better protected to a secondary lethal challenge, a finding with important implications for vaccine development against pathogens that activate NLRC4. Most of the data were generated after performing animal experiments in which wild type and certain NLR- and cytokine-deficient trangenic mice were challenged by Salmonella Typhimurium and their tissues /cells were interrogated ex vivo with microbiological and immunological methods. Some data has been generated by in vitro experiments in which murine bone marrow-derived macrophages were infected with Salmonella Typhimurium and their ability to lyse and secrete cytokines was assessed by classical biochemical and immunological methods.
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Wellcome Trust (108045/Z/15/Z)