Searching for New Z-DNA/Z-RNA Binding Proteins Based on Structural Similarity to Experimentally Validated Zα Domain
| cam.depositDate | 2022-01-13 | |
| cam.issuedOnline | 2022-01-11 | |
| dc.contributor.author | Bartas, Martin | |
| dc.contributor.author | Slychko, Kristyna | |
| dc.contributor.author | Brázda, Václav | |
| dc.contributor.author | Červeň, Jiří | |
| dc.contributor.author | Beaudoin, Christopher | |
| dc.contributor.author | Blundell, Tom | |
| dc.contributor.author | Pečinka, Petr | |
| dc.contributor.orcid | Bartas, Martin [0000-0002-4415-2220] | |
| dc.contributor.orcid | Brázda, Václav [0000-0003-2837-4226] | |
| dc.contributor.orcid | Blundell, Tom [0000-0002-2708-8992] | |
| dc.contributor.orcid | Pečinka, Petr [0000-0003-1270-3646] | |
| dc.date.accessioned | 2022-01-15T00:30:16Z | |
| dc.date.available | 2022-01-15T00:30:16Z | |
| dc.date.issued | 2022-01-11 | |
| dc.date.updated | 2022-01-13T03:57:29Z | |
| dc.description.abstract | <jats:p>Z-DNA and Z-RNA are functionally important left-handed structures of nucleic acids, which play a significant role in several molecular and biological processes including DNA replication, gene expression regulation and viral nucleic acid sensing. Most proteins that have been proven to interact with Z-DNA/Z-RNA contain the so-called Zα domain, which is structurally well conserved. To date, only eight proteins with Zα domain have been described within a few organisms (including human, mouse, Danio rerio, Trypanosoma brucei and some viruses). Therefore, this paper aimed to search for new Z-DNA/Z-RNA binding proteins in the complete PDB structures database and from the AlphaFold2 protein models. A structure-based similarity search found 14 proteins with highly similar Zα domain structure in experimentally-defined proteins and 185 proteins with a putative Zα domain using the AlphaFold2 models. Structure-based alignment and molecular docking confirmed high functional conservation of amino acids involved in Z-DNA/Z-RNA, suggesting that Z-DNA/Z-RNA recognition may play an important role in a variety of cellular processes.</jats:p> | |
| dc.identifier.doi | 10.17863/CAM.80167 | |
| dc.identifier.eissn | 1422-0067 | |
| dc.identifier.issn | 1661-6596 | |
| dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/332726 | |
| dc.language.iso | eng | |
| dc.publisher | MDPI AG | |
| dc.publisher.department | Department of Biochemistry Student | |
| dc.publisher.department | Department of Biochemistry | |
| dc.rights | Attribution 4.0 International | |
| dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
| dc.title | Searching for New Z-DNA/Z-RNA Binding Proteins Based on Structural Similarity to Experimentally Validated Zα Domain | |
| dc.type | Article | |
| dcterms.dateAccepted | 2022-01-05 | |
| prism.endingPage | 768 | |
| prism.issueIdentifier | 2 | |
| prism.publicationName | International Journal of Molecular Sciences | |
| prism.startingPage | 768 | |
| prism.volume | 23 | |
| pubs.licence-display-name | Apollo Repository Deposit Licence Agreement | |
| pubs.licence-identifier | apollo-deposit-licence-2-1 | |
| rioxxterms.type | Journal Article/Review | |
| rioxxterms.version | VoR | |
| rioxxterms.versionofrecord | 10.3390/ijms23020768 |
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