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Research data supporting "Investigation of conventional and unconventional protein secretion using galectin-3"

Repository URI

https://www.repository.cam.ac.uk/handle/1810/317538

Repository DOI

https://doi.org/10.17863/CAM.57330
Thumbnail Image

Files

A1.txt (4.95 MB)
 A2.txt (2.24 MB)
 A3.txt (5.48 MB)
 A4.xlsx (24.73 MB)
 A5.xlsx (104.77 KB)

Type

Dataset

Change log

Authors

Popa, Stephanie  ORCID logo  https://orcid.org/0000-0002-3424-4674

Description

These data files support Stephanie Popa's thesis submitted for the degree of Doctor of Philosophy.

A1: Raw sgRNA count for GeCKOv2 screen (Stewart et al., 2017), .txt file. A2: MaGECK-MLE output for GeCKOv2 screen, .txt file. A3: Raw sgRNA count for Brunello screen, .txt file. A4: MaGECK output for Brunello screen, .xlsx file. A5: siGenome smart pool plate layout, .xlsx file. A6: Luminescence values generated from ss-HRP screen, .txt file. A7: Unprocessed image files generated from Golgi morphology screen, zipped folders (.zip) containing .tif files. A7 has been divided into three folders due to large size (A7-1, A7-2, A7-3).

Version

Software / Usage instructions

MaGECK was used to create A2 and A4: https://sourceforge.net/p/mageck/wiki/Home/

Keywords

CRISPR, CRISPR screen, MaGECK, Galectin-3, Unconventional protein secretion, Protein secretion, Conventional protein secretion, Glycoprotein secretion, siRNA screen, Galectins, GPR161, TMEM220, MARCH2, TMEM199, COL14A1, MXRA7, FAM98B, FAM102B

Publisher

Rights and licensing

Attribution 4.0 International (CC BY 4.0)
Except where otherwised noted, this item's license is described as Attribution 4.0 International (CC BY 4.0)
Sponsorship
Wellcome Trust PhD studentship (109152)

Relationships

Supplements:
https://doi.org/10.17863/CAM.64234

Collections

Research Data - Clinical Biochemistry