Reciprocal Best Structure Hits (RBSH)

Change log
Monzon, Vivian 
Paysan-Lafosse, Typhaine 
Wood, Valerie 
Bateman, Alex 

In this work, we are using AlphaFold structure models to find the closest homologues proteins between Homo sapiens and D. melanogaster, C. elegans, S. cerevisiae and S. pombe as well as between S. cerevisiae and S. pombe. We are using the structure aligner Foldseek to run all against all and search for the best scoring hit in both directions to detect the Reciprocal Best Structure Hits (RBSH). We compare the results to protein pairs detected by their sequence similarity as Reciprocal Best Hits (RBH) and verify the results using the PANTHER family classification files.


Note: This dataset is an updated version of the dataset at

Software / Usage instructions
We used the structure aligner Foldseek to find the RBSH as well as the sequence aligner BLASTP to find the RBH.
AlphaFold database, Protein homology, Schizosaccharomyces pombe, Structure comparison
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