Repository logo
 

Precise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase.

Simple item page
cam.issuedOnline2021-08-05
dc.contributor.authorDavis, Lloyd
dc.contributor.authorRadman, Inja
dc.contributor.authorGoutou, Angeliki
dc.contributor.authorTynan, Ailish
dc.contributor.authorBaxter, Kieran
dc.contributor.authorXi, Zhiyan
dc.contributor.authorO'Shea, Jack M
dc.contributor.authorChin, Jason W
dc.contributor.authorGreiss, Sebastian
dc.contributor.orcidO'Shea, Jack M [0000-0002-9694-7340]
dc.contributor.orcidGreiss, Sebastian [0000-0001-9130-0831]
dc.date.accessioned2021-10-26T23:30:27Z
dc.date.available2021-10-26T23:30:27Z
dc.date.issued2021-08-05
dc.description.abstractSynthetic strategies for optically controlling gene expression may enable the precise spatiotemporal control of genes in any combination of cells that cannot be targeted with specific promoters. We develop an improved genetic code expansion system in Caenorhabditis elegans and use it to create a photoactivatable Cre recombinase. We laser-activate Cre in single neurons within a bilaterally symmetric pair to selectively switch on expression of a loxP-controlled optogenetic channel in the targeted neuron. We use the system to dissect, in freely moving animals, the individual contributions of the mechanosensory neurons PLML/PLMR to the C. elegans touch response circuit, revealing distinct and synergistic roles for these neurons. We thus demonstrate how genetic code expansion and optical targeting can be combined to break the symmetry of neuron pairs and dissect behavioural outputs of individual neurons that cannot be genetically targeted.
dc.format.mediumElectronic
dc.identifier.doi10.17863/CAM.77360
dc.identifier.eissn2050-084X
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/329917
dc.languageeng
dc.language.isoeng
dc.publishereLife Sciences Publications, Ltd
dc.publisher.urlhttp://dx.doi.org/10.7554/elife.67075
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectC. elegans
dc.subjectCre recombinase
dc.subjectbiochemistry
dc.subjectchemical biology
dc.subjectgenetic code expansion
dc.subjectneuroscience
dc.subjectnon canonical amino acids
dc.subjectoptogenetics
dc.subjectphoto-caged amino acid
dc.subjectAnimals
dc.subjectBehavior, Animal
dc.subjectCaenorhabditis elegans
dc.subjectGene Expression
dc.subjectGenetic Code
dc.subjectGenetic Engineering
dc.subjectIntegrases
dc.subjectNeurons
dc.subjectOptogenetics
dc.subjectTouch Perception
dc.titlePrecise optical control of gene expression in C elegans using improved genetic code expansion and Cre recombinase.
dc.typeArticle
dcterms.dateAccepted2021-08-04
prism.publicationDate2021
prism.publicationNameElife
prism.volume10
rioxxterms.licenseref.startdate2021-08-05
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.7554/eLife.67075

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Precise optical control of gene expression in iC elegansi using improved genetic code expansion and Cre recombinase.pdf
Size:
3.86 MB
Format:
Adobe Portable Document Format
Description:
Published version
Licence
https://creativecommons.org/licenses/by/4.0/
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
DepositLicenceAgreementv2.1.pdf
Size:
150.9 KB
Format:
Adobe Portable Document Format
Download

Collections

Cambridge University Research Outputs