Data to support paper "Organization and triggered release of liposomes with DNA-based synthetic condensates". Each folder for main figures (1–5) is accompanied by a corresponding README file.

1. Confocal micrographs of a hybrid DNA condensate incorporating lipid vesicles (LUVs); dynamic light scattering size distributions and polydispersity indices for LUVs with varying cholesterol anchor densities; and confocal micrographs and plate reader fluorescence data for a calcein retention and release assay.

2. Confocal micrographs of DNA condensates with internally-sequestered or surface-tethered LUVs; radial intensity profiles quantifying LUV localisation within condensates; per-condensate surface-to-core LUV fluorescence ratios and theoretical binding model predictions as a function of cholesterol anchor number; and confocal micrographs demonstrating orthogonal, sequence-selective LUV targeting in mixed A+B condensate systems.

3. Confocal micrographs and per-channel grayscale image montages of biphasic A/B DNA condensates with LUVs across a range of cross-linker fractions (F_ab); per-condensate 2D correlation coefficients between LUV signal and condensate phase masks, and partition coefficient measurements; and control micrographs of condensates without LUVs and of bi-functional LUVs acting as linkers between phases.

4. Epifluorescence timelapse micrographs of condensate disassembly upon sequence-specific trigger strand addition; time-resolved coefficient of variation and 2D LUV–phase correlation coefficients across three independent replicates; and epifluorescence micrographs showing condensate response to individual and combined trigger strands.

5. Epifluorescence timelapse micrographs and per-ROI fluorescence intensity time-series tracking the triggered release of LUVs from DNA sender condensates and their capture by amphiphilic crystalline receiver condensates; confocal micrographs and 3D reconstructions of amphiphilic condensates coated with captured LUVs, including at 6 days post-trigger; and confocal micrographs of HEK293 cells co-incubated with DNA condensates and calcein-loaded LUVs, imaged immediately after addition and 24 h later.