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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Azuma, Rika 
Miyamoto, Kei 
Oikawa, Mami 
Yamada, Masayasu 
Anzai, Masayuki 

Abstract

Somatic cell nuclear transfer (SCNT) provides a unique opportunity to directly produce a cloned animal from a donor cell, and it requires the use of skillful techniques. Additionally, the efficiencies of cloning have remained low since the successful production of cloned animals, especially mice. There have been many attempts to improve the cloning efficiency, and trichostatin A (TSA), a histone deacetylase inhibitor, has been widely used to enhance the efficiency of cloning. Here, we report a dramatically improved cloning method in mice. This somatic cell nuclear transfer method involves usage of Hemagglutinating virus of Japan Envelope (HVJ-E), which enables easy manipulation. Moreover, the treatment using two small molecules, TSA and vitamin C (VC), with deionized bovine serum albumin (dBSA), is highly effective for embryonic development. This approach requires neither additional injection nor genetic manipulation, and thus presents a simple, suitable method for practical use. This method could become a technically feasible approach for researchers to produce genetically modified animals from cultured cells. Furthermore, it might be a useful way for the rescue of endangered animals via cloning.

Description

Keywords

Animals, Ascorbic Acid, Cloning, Organism, Embryonic Development, Female, Hydroxamic Acids, Male, Mice, Nuclear Transfer Techniques, Pregnancy

Journal Title

J Vis Exp

Conference Name

Journal ISSN

1940-087X
1940-087X

Volume Title

Publisher

MyJove Corporation
Sponsorship
Wellcome Trust (203144/Z/16/Z)
Cancer Research UK (C6946/A24843)