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dc.contributor.authorGenkov, Todor
dc.contributor.authorMeyer, Moritz
dc.contributor.authorGriffiths, Howard
dc.contributor.authorSpreitzer, Robert J
dc.date.accessioned2010-10-05T09:01:00Z
dc.date.available2010-10-05T09:01:00Z
dc.date.issued2010-06-25
dc.identifier.citationTHE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 285, NO. 26, pp. 19833–19841, June 25, 2010
dc.identifier.issn0021-9258
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/226623
dc.description.abstractThere has been much interest in the chloroplast-encoded large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in net CO(2) fixation in photosynthesis. Improvements in the enzyme would lead to an increase in the production of food, fiber, and renewable energy. Although the large subunit contains the active site, a family of rbcS nuclear genes encodes the Rubisco small subunits, which can also influence the carboxylation catalytic efficiency and CO(2)/O(2) specificity of the enzyme. To further define the role of the small subunit in Rubisco function, small subunits from spinach, Arabidopsis, and sunflower were assembled with algal large subunits by transformation of a Chlamydomonas reinhardtii mutant that lacks the rbcS gene family. Foreign rbcS cDNAs were successfully expressed in Chlamydomonas by fusing them to a Chlamydomonas rbcS transit peptide sequence engineered to contain rbcS introns. Although plant Rubisco generally has greater CO(2)/O(2) specificity but a lower carboxylation V(max) than Chlamydomonas Rubisco, the hybrid enzymes have 3-11% increases in CO(2)/O(2) specificity and retain near normal V(max) values. Thus, small subunits may make a significant contribution to the overall catalytic performance of Rubisco. Despite having normal amounts of catalytically proficient Rubisco, the hybrid mutant strains display reduced levels of photosynthetic growth and lack chloroplast pyrenoids. It appears that small subunits contain the structural elements responsible for targeting Rubisco to the algal pyrenoid, which is the site where CO(2) is concentrated for optimal photosynthesis.
dc.description.sponsorshipThis work was supported in part by Grant DE-FG02-00ER15044 from the United States Department of Energy.
dc.languageen_US
dc.language.isoen_US
dc.publisherElsevier BV
dc.titleFunctional hybrid rubisco enzymes with plant small subunits and algal large subunits: engineered rbcS cDNA for expression in chlamydomonas.
dc.typeArticle
prism.publicationDate2010
prism.publicationNameJ Biol Chem
rioxxterms.versionofrecord10.1074/jbc.M110.124230
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2010-04-27
dc.contributor.orcidGriffiths, Howard [0000-0002-3009-6563]
dc.identifier.eissn1083-351X
rioxxterms.typeJournal Article/Review


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