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dc.contributor.authorAvril-Sassen, Stefanie
dc.contributor.authorGoldstein, Leonard
dc.contributor.authorStingl, John
dc.contributor.authorBlenkiron, Cherie
dc.contributor.authorLe Quesne, John
dc.contributor.authorSpiteri, Inmaculada
dc.contributor.authorKaragavriilidou, Konstantina
dc.contributor.authorWatson, Christine
dc.contributor.authorTavaré, Simon
dc.contributor.authorMiska, Eric
dc.contributor.authorCaldas, Carlos
dc.date.accessioned2011-06-14T16:14:51Z
dc.date.available2011-06-14T16:14:51Z
dc.date.issued2009-11-20
dc.identifier.citationBMC Genomics 2009, 10:548
dc.identifier.issn1471-2164
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237716
dc.description.abstractBACKGROUND: The differential expression pattern of microRNAs (miRNAs) during mammary gland development might provide insights into their role in regulating the homeostasis of the mammary epithelium. Our aim was to analyse these regulatory functions by deriving a comprehensive tissue-specific combined miRNA and mRNA expression profile of post-natal mouse mammary gland development.We measured the expression of 318 individual murine miRNAs by bead-based flow-cytometric profiling of whole mouse mammary glands throughout a 16-point developmental time course, including juvenile, puberty, mature virgin, gestation, lactation, and involution stages. In parallel whole-genome mRNA expression data were obtained. RESULTS: One third (n = 102) of all murine miRNAs analysed were detected during mammary gland development. MicroRNAs were represented in seven temporally co-expressed clusters, which were enriched for both miRNAs belonging to the same family and breast cancer-associated miRNAs. Global miRNA and mRNA expression was significantly reduced during lactation and the early stages of involution after weaning. For most detected miRNA families we did not observe systematic changes in the expression of predicted targets. For miRNA families whose targets did show changes, we observed inverse patterns of miRNA and target expression. The data sets are made publicly available and the combined expression profiles represent an important community resource for mammary gland biology research. CONCLUSION: MicroRNAs were expressed in likely co-regulated clusters during mammary gland development. Breast cancer-associated miRNAs were significantly enriched in these clusters. The mechanism and functional consequences of this miRNA co-regulation provide new avenues for research into mammary gland biology and generate candidates for functional validation.
dc.languageEnglish
dc.language.isoen
dc.publisherSpringer Science and Business Media LLC
dc.titleCharacterisation of microRNA expression in post-natal mouse mammary gland development.
dc.typeArticle
dc.date.updated2011-06-14T16:14:51Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.
dc.rights.holderAvril-Sassen et al.; licensee BioMed Central Ltd.
prism.publicationDate2009
prism.publicationNameBMC Genomics
dcterms.dateAccepted2009-11-20
rioxxterms.versionofrecord10.1186/1471-2164-10-548
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2009-11-20
dc.contributor.orcidWatson, Christine [0000-0002-8548-5902]
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.contributor.orcidCaldas, Carlos [0000-0003-3547-1489]
dc.identifier.eissn1471-2164
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMRC (G0300723B)
pubs.funder-project-idBreast Cancer Now (None)
pubs.funder-project-idBreast Cancer Campaign (2005NOV44)
cam.issuedOnline2009-11-20


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