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dc.contributor.authorBarraud, Perrineen
dc.contributor.authorHe, Xiaolingen
dc.contributor.authorCaldwell, Maeveen
dc.contributor.authorFranklin, Robinen
dc.date.accessioned2011-06-16T16:30:29Z
dc.date.available2011-06-16T16:30:29Z
dc.date.issued2008-02-18en
dc.identifier.citationBMC Neuroscience 2008, 9:24
dc.identifier.issn1471-2202
dc.identifier.urihttp://www.dspace.cam.ac.uk/handle/1810/237989
dc.description.abstractAbstract Background The olfactory epithelium is a neurogenic tissue comprising a population of olfactory receptor neurons that are renewed throughout adulthood by a population of stem and progenitor cells. Because of their relative accessibility compared to intra-cranially located neural stem/progenitor cells, olfactory epithelium stem and progenitor cells make attractive candidates for autologous cell-based therapy. However, olfactory stem and progenitor cells expand very slowly when grown as free-floating spheres (olfactory-spheres) under growth factor stimulation in a neurosphere assay. Results In order to address whether olfactory mucosa cells extrinsically regulate proliferation and/or differentiation of immature neural cells, we cultured neural progenitor cells derived from mouse neonatal olfactory bulb or subventricular zone (SVZ) in the presence of medium conditioned by olfactory mucosa-derived spheres (olfactory-spheres). Our data demonstrated that olfactory mucosa cells produced soluble factors that affect bulbar neural progenitor cell differentiation but not their proliferation when compared to control media. In addition, olfactory mucosa derived soluble factors increased neurogenesis, especially favouring the generation of non-GABAergic neurons. Olfactory mucosa conditioned medium also contained several factors with neurotrophic/neuroprotective properties. Olfactory-sphere conditioned medium did not affect proliferation or differentiation of SVZ-derived neural progenitors. Conclusion These data suggest that the olfactory mucosa does not contain factors that are inhibitory to neural stem/progenitor cell proliferation but does contain factors that steer differentiation toward neuronal phenotypes. Moreover, they suggest that the poor expansion of olfactory-spheres may be in part due to intrinsic properties of the olfactory epithelial stem/progenitor cell population.
dc.languageEnglishen
dc.language.isoen
dc.titleSecreted factors from olfactory mucosa cells expanded as free-floating spheres increase neurogenesis in olfactory bulb neurosphere culturesen
dc.typeArticle
dc.date.updated2011-06-16T16:30:29Z
dc.description.versionRIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.en
dc.rights.holderBarraud et al.; licensee BioMed Central Ltd.
prism.publicationDate2008en
dcterms.dateAccepted2008-02-18en
rioxxterms.versionofrecord10.1186/1471-2202-9-24en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2008-02-18en
dc.contributor.orcidFranklin, Robin [0000-0001-6522-2104]
dc.identifier.eissn1471-2202
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (G0300338)
pubs.funder-project-idMRC (G0300723B)


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