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dc.contributor.authorTrinh, Anneen
dc.contributor.authorRye, Ingaen
dc.contributor.authorAlmendro, Vanessaen
dc.contributor.authorHelland, Åslaugen
dc.contributor.authorRussnes, Hegeen
dc.contributor.authorMarkowetz, Florianen
dc.date.accessioned2014-09-10T13:56:53Z
dc.date.available2014-09-10T13:56:53Z
dc.date.issued2014-08-26en
dc.identifier.citation(2014) Genome Biology 15: 422en
dc.identifier.issn1474-760X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/245902
dc.description.abstractMolecular analysis has revealed extensive intra-tumor heterogeneity in human cancer samples, but cannot identify cell-to-cell variations within the tissue microenvironment. In contrast, in situ analysis can identify genetic aberrations in phenotypically defined cell subpopulations while preserving tissue-context specificity. GoIFISH is a widely applicable, user-friendly system tailored for the objective and semi-automated visualization, detection and quantification of genomic alterations and protein expression obtained from fluorescence in situ analysis. In a sample set of HER2-positive breast cancers GoIFISH is highly robust in visual analysis and its accuracy compares favorably to other leading image analysis methods. GoIFISH is freely available at www.sourceforge.net/projects/goifish/
dc.languageen_USen
dc.language.isoen_USen
dc.publisherBioMed Central
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.titleGoIFISH: a system for the quantification of single cell heterogeneity from IFISH imagesen
dc.typeArticle
dc.description.versionThis is the final published version. It is also available from Genome Biology at http://genomebiology.com/2014/15/8/442.en
prism.publicationDate2014en
prism.publicationNameGenome Biologyen
prism.startingPage442
prism.volume15en
dc.rioxxterms.funderCancer Research UK Hutchinson Whampoa Limited Helse Sør-Øst The Norwegian Cancer Association K. G. Jebsen Foundation Radiumhospitalets legater Breast Cancer Research Foundation National Cancer Institute
dcterms.dateAccepted2014-08-15en
rioxxterms.versionofrecord10.1186/s13059-014-0442-yen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-08-26en
dc.contributor.orcidMarkowetz, Florian [0000-0002-2784-5308]
dc.identifier.eissn1474-760X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (C14303_do not transfer)
pubs.funder-project-idCancer Research UK (CB4320)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales