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dc.contributor.authorPatrick, P Stephenen
dc.contributor.authorRodrigues, Tiago Ben
dc.contributor.authorKettunen, Mikko Ien
dc.contributor.authorLyons, Scott Ken
dc.contributor.authorNeves, Andreen
dc.contributor.authorBrindle, Kevinen
dc.date.accessioned2015-06-22T11:12:59Z
dc.date.available2015-06-22T11:12:59Z
dc.date.issued2015-05-15en
dc.identifier.citationMagnetic Resonance in Medicine 2015 DOI: 10.1002/mrm.25750en
dc.identifier.issn0740-3194
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248607
dc.description.abstractPurpose: To assess the potential of an MRI gene reporter based on the ferritin receptor Timd2 (T-cell immunoglobulin and mucin domain containing protein 2), using T1- and T2- weighted imaging. Methods: Pellets of cells that had been modified to express the Timd2 transgene, and incubated with either iron-loaded or manganese-loaded ferritin, were imaged using T1- and T2- weighted MRI. Mice were also implanted subcutaneously with Timd2-expressing cells and the resulting xenograft tissue imaged following intravenous injection of ferritin using T2- weighted imaging. Results: Timd2-expressing cells, but not control cells, showed a large increase in both R2 and R1 in vitro following incubation with iron-loaded and manganese-loaded ferritin, respectively. Expression of Timd2 had no effect on cell viability or proliferation; however, manganese-loaded ferritin, but not iron-loaded ferritin, was toxic to Timd2-expressing cells. Timd2-expressing xenografts in vivo showed much smaller changes in R2 following injection of iron-loaded ferritin than the same cells incubated in vitro with iron-loaded ferritin. Conclusion: Timd2 has demonstrated potential as an MRI reporter gene, producing large increases in R2 and R1 with ferritin and manganese-loaded ferritin respectively in vitro, although more modest changes in R2 in vivo. Manganese-loaded apoferritin was not used in vivo due to the toxicity observed in vitro.
dc.description.sponsorshipThis work was supported by the Medical Research Council and Cancer Research UK (CRUK) doctoral training grants (to P.S.P.) and a CRUK Program Grant to K.M.B. T.B.R. was in receipt of Intra-European Marie Curie and Long-Term European Molecular Biology Organization fellowships.
dc.languageEnglishen
dc.language.isoenen
dc.publisherWiley on behalf of International Society for Magnetic Resonance in Medicine.
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.subjectferritinen
dc.subjectmanganeseen
dc.subjectT1-weighteden
dc.subjectT2-weighteden
dc.subjectreporter geneen
dc.titleDevelopment of Timd2 as a Reporter Gene for MRIen
dc.typeArticle
dc.description.versionThis is the final published version. It first appeared at http://onlinelibrary.wiley.com/doi/10.1002/mrm.25750/abstract.en
prism.publicationDate2015en
prism.publicationNameMagnetic Resonance in Medicineen
dc.rioxxterms.funderMRC
dc.rioxxterms.funderCRUK
dcterms.dateAccepted2015-03-27en
rioxxterms.versionofrecord10.1002/mrm.25750en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-05-15en
dc.contributor.orcidNeves, Andre [0000-0003-2740-5166]
dc.contributor.orcidBrindle, Kevin [0000-0003-3883-6287]
dc.identifier.eissn1522-2594
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (16465)
pubs.funder-project-idCancer Research UK (CB4100)
pubs.funder-project-idCancer Research UK (C14303_do not transfer)


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Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales