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dc.contributor.authorCutler, Nicken
dc.contributor.authorChaput, Dominique Len
dc.contributor.authorOliver, Anna Een
dc.contributor.authorViles, Heather Aen
dc.date.accessioned2015-06-25T11:40:25Z
dc.date.available2015-06-25T11:40:25Z
dc.date.issued2014-12-15en
dc.identifier.citationFEMS Microbiology Ecology 2015. doi: 10.1093/femsec/fiu027en
dc.identifier.issn0168-6496
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248705
dc.description.abstractMicrobial biofilms are common on lithic surfaces, including stone buildings. However, the ecology of these communities is poorly understood. Few studies have focused on the spatial characteristics of lithobiontic biofilms, despite the fact that spatial structure has been demonstrated to influence ecosystem function (and hence biodegradation) and community diversity. Furthermore, relatively few studies have utilized molecular techniques to characterize these communities, even though molecular methods have revealed unexpected microbial diversity in other habitats. This study investigated (1) the spatial structure and (2) the taxonomic composition of an epilithic biofilm using molecular techniques, namely amplicon pyrosequencing and terminal restriction fragment length polymorphism. Dispersion indices and Mantel correlograms were used to test for the presence of spatial structure in the biofilm. Diversity metrics and rank-abundance distributions (RADs) were also generated. The study revealed spatial structure on a centimetre scale in eukaryotic microbes (fungi and algae), but not the bacteria. Fungal and bacterial communities were highly diverse; algal communities much less so. The RADs were characterized by a distinctive 'hollow' (concave up) profile and long tails of rare taxa. These findings have implications for understanding the ecology of epilithic biofilms and the spatial heterogeneity of stone biodeterioration.
dc.description.sponsorshipThis work was supported by the Engineering and Physical Sciences Research Council (grant no. EP/G011338/1).
dc.languageEnglishen
dc.language.isoenen
dc.publisherOUP
dc.subjectamplicon pyrosequencingen
dc.subjectTRFLPen
dc.subjectlithobiontic microbesen
dc.subjectgreen algaeen
dc.subjectbacteriaen
dc.subjectfungien
dc.subjectmantel correlogramsen
dc.subjectrank-abundance distributionsen
dc.titleThe spatial organization and microbial community structure of an epilithic biofilmen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from OUP at http://femsec.oxfordjournals.org/content/91/3/fiu027.en
prism.numberfiu027en
prism.publicationDate2014en
prism.publicationNameFEMS Microbiology Ecologyen
prism.volume91en
dc.rioxxterms.funderEPSRC
dc.rioxxterms.projectidEP/G011338/1
dcterms.dateAccepted2014-12-10en
rioxxterms.versionofrecord10.1093/femsec/fiu027en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2014-12-15en
dc.contributor.orcidCutler, Nick [0000-0003-1746-7769]
dc.identifier.eissn1574-6941
rioxxterms.typeJournal Article/Reviewen
rioxxterms.freetoread.startdate2015-12-15


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