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dc.contributor.authorKieckbusch, Jensen
dc.contributor.authorGaynor, Louise Men
dc.contributor.authorColucci, Francescoen
dc.date.accessioned2015-07-01T13:44:04Z
dc.date.available2015-07-01T13:44:04Z
dc.date.issued2015-12-05en
dc.identifier.citationJournal of Visualized Experiments 2015, 106: e53534. doi:10.3791/53534en
dc.identifier.issn1940-087X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248780
dc.description.abstractThe placenta mediates the exchange of factors such as gases and nutrients between mother and fetus and has specific demands for supply of blood from the maternal circulation. The maternal uterine vasculature needs to adapt to this temporary demand and the success of this arterial remodeling process has implications for fetal growth. Cells of the maternal immune system, especially natural killer (NK) cells, play a critical role in this process. Here we describe a method to assess the degree of remodeling of maternal spiral arteries during mouse pregnancy. Hematoxylin and eosin-stained tissue sections are scanned and the size of the vessels analysed. As a complementary validation method, we also present a qualitative assessment for the success of the remodeling process by immunohistochemical detection of smooth muscle actin (SMA), which normally disappears from within the arterial vascular media at mid-gestation. Together, these methods enable determination of an important parameter of the pregnancy phenotype. These results can be combined with other endpoints of mouse pregnancy to provide insight into the mechanisms underlying pregnancy-related complications.
dc.description.sponsorshipThis work was funded by The Wellcome Trust [094073/Z/10/Z], The Centre for Trophoblast Research and The British Heart Foundation.
dc.languageEnglishen
dc.language.isoenen
dc.publisherJOVE
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 4.0 International License
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectReproductionen
dc.subjectVasculatureen
dc.subjectImmunologyen
dc.subjectNK cellsen
dc.subjectMouseen
dc.subjectStereologyen
dc.subjectImmunohistochemistryen
dc.subjectPregnancyen
dc.titleAssessment of maternal vascular remodeling during pregnancy in the mouse uterusen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from JOVE via http://dx.doi.org/10.3791/53534en
prism.number2015en
prism.publicationDate2015en
prism.publicationNameJournal of Visualized Experimentsen
prism.volume106en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderBHF
dc.rioxxterms.projectid094073/Z/10/Z
rioxxterms.versionofrecord10.3791/53534en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-12-05en
dc.contributor.orcidKieckbusch, Jens [0000-0002-5930-1575]
dc.contributor.orcidColucci, Francesco [0000-0001-5193-6376]
dc.identifier.eissn1940-087X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBritish Heart Foundation (FS/12/4/29254)
pubs.funder-project-idWellcome Trust (094073/Z/10/Z)


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Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License
Except where otherwise noted, this item's licence is described as Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License