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dc.contributor.authorFonville, Judithen
dc.contributor.authorFraaij, Pieter LAen
dc.contributor.authorde, Mutsert Gerrieen
dc.contributor.authorWilks, Samuel Hen
dc.contributor.authorvan, Beek Ruuden
dc.contributor.authorFouchier, Ron AMen
dc.contributor.authorRimmelzwaan, Guus Fen
dc.date.accessioned2015-07-02T13:25:15Z
dc.date.available2015-07-02T13:25:15Z
dc.date.issued2015-07-03en
dc.identifier.citationThe Journal of Infectious Diseases 2015, 213(1): 31-38. doi:10.1093/infdis/jiv367en
dc.identifier.issn0022-1899
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248820
dc.description.abstractBackground: Antigenic characterization of influenza viruses is typically based on hemagglutination inhibition (HI) assay data of viral isolates tested against strain-specific post-infection ferret antisera. Here, similar virus characterizations were performed using first-infection human rather than ferret serology data. Methods: We screened sera collected between 1995 and 2011 from children between 9 and 24 months of age for influenza virus antibodies, performed HI tests for the positive sera against 24 influenza viruses isolated between 1989 and 2011, and measured HI titers of 24 A/H3N2 ferret sera against the same panel of viruses. Results: Of the 17 positive human sera, 6 were high-responders, showing HI patterns that would be expected from primary infection antisera, while 11 sera had lower, more dispersed patterns of reactivity that are not easily explained. The antigenic map based on the high-responder human HI data was similar to the results using ferret data. Conclusions: Although the overall structure of the ferret and human antigenic maps is similar, local differences in virus positions indicate that the human and ferret immune system might see antigenic properties of viruses differently. Further studies are needed to establish to what degree of detail ferret data provide equivalent patterns to human serological reactivity.
dc.description.sponsorshipThis work was supported by the award of a Fellowship in Biomedical Informatics from the Medical Research Council UK [grant number MR/K021885/1] and a Junior Research Fellowship from Homerton College Cambridge to J.M.F.; a Medical Research Council UK studentship [number MR/K50127X/1 to S.H.W.]; the EU FP7 project PREPARE [grant number 602525 to P.L.A.F.]; the National Institute of Allergy and Infectious Diseases, National Institutes of Health [contract number HHSN272201400008C to R.A.M.F and the Center for Pathogen Evolution]; and the EU grant FLUNIVAC [grant number 602604 to G.F.R.].
dc.languageEnglishen
dc.language.isoenen
dc.publisherOxford Journals
dc.rightsAttribution 4.0
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPrimary infectionen
dc.subjectinfluenzaen
dc.subjecthuman antiseraen
dc.subjectantigenic cartographyen
dc.subjectantibody landscapesen
dc.titleAntigenic maps of influenza A/H3N2 virus produced with human antisera obtained after primary infectionen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Oxford University Press via http://dx.doi.org/10.1093/infdis/jiv367en
prism.endingPage38
prism.publicationDate2015en
prism.publicationNameThe Journal of Infectious Diseasesen
prism.startingPage31
prism.volume213en
dc.rioxxterms.funderMRC
dc.rioxxterms.funderEU FP7
dc.rioxxterms.projectidMR/K021885/1
dc.rioxxterms.projectidMR/K50127X/1
dc.rioxxterms.projectid602525
dc.rioxxterms.projectid602604
dcterms.dateAccepted2015-06-25en
rioxxterms.versionofrecord10.1093/infdis/jiv367en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-07-03en
dc.identifier.eissn1537-6613
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MR/K021885/1)
pubs.funder-project-idNational Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)


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Attribution 4.0
Except where otherwise noted, this item's licence is described as Attribution 4.0