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dc.contributor.authorFonville, Judith M
dc.contributor.authorFraaij, Pieter LA
dc.contributor.authorde Mutsert, Gerrie
dc.contributor.authorWilks, Samuel H
dc.contributor.authorvan Beek, Ruud
dc.contributor.authorFouchier, Ron AM
dc.contributor.authorRimmelzwaan, Guus F
dc.date.accessioned2015-07-02T13:25:15Z
dc.date.available2015-07-02T13:25:15Z
dc.date.issued2016-01-01
dc.identifier.citationThe Journal of Infectious Diseases 2015, 213(1): 31-38. doi:10.1093/infdis/jiv367
dc.identifier.issn0022-1899
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/248820
dc.description.abstractBACKGROUND: Antigenic characterization of influenza viruses is typically based on hemagglutination inhibition (HI) assay data for viral isolates tested against strain-specific postinfection ferret antisera. Here, similar virus characterizations were performed using serological data from humans with primary influenza A(H3N2) infection. METHODS: We screened sera collected between 1995 and 2011 from children between 9 and 24 months of age for influenza virus antibodies, performed HI tests for the positive sera against 23 influenza viruses isolated between 1989 and 2011, and measured HI titers of antisera against influenza A(H3N2) from 24 ferrets against the same panel of viruses. RESULTS: Of the 17 positive human sera, 6 had a high response, showing HI patterns that would be expected from primary infection antisera, while 11 sera had lower, more dispersed patterns of reactivity that are not easily explained. The antigenic map based on the high-response human HI data was similar to the map created using ferret data. CONCLUSIONS: Although the overall structure of the ferret and human antigenic maps is similar, local differences in virus positions indicate that the human and ferret immune system might see antigenic properties of viruses differently. Further studies are needed to establish the degree of similarity between serological patterns in ferret and human data.
dc.description.sponsorshipThis work was supported by the award of a Fellowship in Biomedical Informatics from the Medical Research Council UK [grant number MR/K021885/1] and a Junior Research Fellowship from Homerton College Cambridge to J.M.F.; a Medical Research Council UK studentship [number MR/K50127X/1 to S.H.W.]; the EU FP7 project PREPARE [grant number 602525 to P.L.A.F.]; the National Institute of Allergy and Infectious Diseases, National Institutes of Health [contract number HHSN272201400008C to R.A.M.F and the Center for Pathogen Evolution]; and the EU grant FLUNIVAC [grant number 602604 to G.F.R.].
dc.languageEnglish
dc.language.isoen
dc.publisherOxford University Press (OUP)
dc.rightsAttribution 4.0
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPrimary infection
dc.subjectinfluenza
dc.subjecthuman antisera
dc.subjectantigenic cartography
dc.subjectantibody landscapes
dc.titleAntigenic Maps of Influenza A(H3N2) Produced With Human Antisera Obtained After Primary Infection.
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Oxford University Press via http://dx.doi.org/10.1093/infdis/jiv367
prism.endingPage38
prism.publicationDate2015
prism.publicationNameJ Infect Dis
prism.startingPage31
prism.volume213
dc.rioxxterms.funderMRC
dc.rioxxterms.funderEU FP7
dc.rioxxterms.projectidMR/K021885/1
dc.rioxxterms.projectidMR/K50127X/1
dc.rioxxterms.projectid602525
dc.rioxxterms.projectid602604
dcterms.dateAccepted2015-06-25
rioxxterms.versionofrecord10.1093/infdis/jiv367
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-07-03
dc.identifier.eissn1537-6613
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MR/K021885/1)
pubs.funder-project-idNational Institutes of Health (NIH) (via Mount Sinai School of Medicine (MSSM)) (HHSN272201400008C)
cam.issuedOnline2015-07-03


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Attribution 4.0
Except where otherwise noted, this item's licence is described as Attribution 4.0