Show simple item record

dc.contributor.authorLim, Kennethen
dc.contributor.authorGroen, Arnouden
dc.contributor.authorMolostvov, Guermanen
dc.contributor.authorLu, Tzongshien
dc.contributor.authorLilley, Kathrynen
dc.contributor.authorSnead, Daviden
dc.contributor.authorJames, Seanen
dc.contributor.authorWilkinson, Ianen
dc.contributor.authorTing, Stephenen
dc.contributor.authorHsiao, Li-Lien
dc.contributor.authorHiemstra, Thomasen
dc.contributor.authorZehnder, Danielen
dc.date.accessioned2015-08-25T12:17:43Z
dc.date.available2015-08-25T12:17:43Z
dc.date.issued2015-08-17en
dc.identifier.citationJournal of Clinical Endocrinology & Metabolism 2015, 100(10):E1308 –E1318. doi:10.1210/jc.2015-1800en
dc.identifier.issn0021-972X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/250359
dc.description.abstractContext: α-Klotho has emerged as a powerful regulator of the aging process. To-date, the expression profile of α-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane α-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human α-Klotho expression in health. Results: The distribution of α-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong α-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, α-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane α-Klotho isoform. Conclusions: The data presented confirms α-Klotho expression in the kidney tubule and in artery, and provides evidence of α-Klotho expression across organ systems and cell-types that have not previously been described in humans.
dc.description.sponsorshipK.L. received a Genzyme-Sanofi Fellowship in Nephrology grant. T.F.H. is funded by the NIHR award to the Cambridge Biomedical Research Centre and by NIHR grant 14/49/147. The Cambridge Aorta Study is funded by the British Heart Foundation.
dc.languageEnglishen
dc.language.isoenen
dc.publisherEndocrine Society
dc.subjectKlothoen
dc.subjectProteomicsen
dc.subjectExtrarenalen
dc.titleα-Klotho expressison in human tissueen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from the Endocrine Society via http://dx.doi.org/10.1210/jc.2015-1800.en
prism.endingPageE1318
prism.publicationDate2015en
prism.publicationNameJournal of Clinical Endocrinology & Metabolismen
prism.startingPageE1308
prism.volume100en
dc.rioxxterms.funderBHF
dc.rioxxterms.funderNIHR
dc.rioxxterms.projectid14/49/147
rioxxterms.versionofrecord10.1210/jc.2015-1800en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-08-17en
dc.contributor.orcidLilley, Kathryn [0000-0003-0594-6543]
dc.contributor.orcidWilkinson, Ian [0000-0001-6598-9399]
dc.contributor.orcidHiemstra, Thomas [0000-0002-2115-8689]
dc.identifier.eissn1945-7197
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBritish Heart Foundation (FS/12/8/29377)


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record