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dc.contributor.authorGorshkova, Tatyanaen
dc.contributor.authorMokshina, Nataliaen
dc.contributor.authorChernova, Tatyanaen
dc.contributor.authorIbragimova, Nadezhdaen
dc.contributor.authorSalnikov, Vadimen
dc.contributor.authorMikshina, Polinaen
dc.contributor.authorTryfona, Theodoraen
dc.contributor.authorBanasiak, Alicjaen
dc.contributor.authorImmerzeel, Peteren
dc.contributor.authorDupree, Paulen
dc.contributor.authorMellerowicz, Ewa Jen
dc.date.accessioned2015-12-15T15:14:32Z
dc.date.available2015-12-15T15:14:32Z
dc.date.issued2015-09-16en
dc.identifier.citationGorshkova et al. Plant Physiology (2015) Vol. 169 No. 3, pp. 2048-2063. doi: 10.​1104/​pp.​15.​00690en
dc.identifier.issn0032-0889
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/252983
dc.description.abstractContractile cell walls are found in various plant organs and tissues such as tendrils, contractile roots, and tension wood. The tension-generating mechanism is not known but is thought to involve special cell wall architecture. We previously postulated that tension could result from the entrapment of certain matrix polymers within cellulose microfibrils. As reported here, this hypothesis was corroborated by sequential extraction and analysis of cell wall polymers that are retained by cellulose microfibrils in tension wood and normal wood of hybrid aspen (Populus tremula × Populus tremuloides). β-(1→4)-Galactan and type II arabinogalactan were the main large matrix polymers retained by cellulose microfibrils that were specifically found in tension wood. Xyloglucan was detected mostly in oligomeric form in the alkali-labile fraction and was enriched in tension wood. β-(1→4)-Galactan and rhamnogalacturonan I backbone epitopes were localized in the gelatinous cell wall layer. Type II arabinogalactans retained by cellulose microfibrils had a higher content of (methyl)glucuronic acid and galactose in tension wood than in normal wood. Thus, β-(1→4)-galactan and a specialized form of type II arabinogalactan are trapped by cellulose microfibrils specifically in tension wood and, thus, are the main candidate polymers for the generation of tensional stresses by the entrapment mechanism. We also found high β-galactosidase activity accompanying tension wood differentiation and propose a testable hypothesis that such activity might regulate galactan entrapment and, thus, mechanical properties of cell walls in tension wood.
dc.description.sponsorshipThis work was supported by the Swedish Governmental Agency for Innovation Systems, the Swedish Research Council, the Russian Foundation for Basic Research (grant nos. 15–04–02560 and 15–04–05721), and the Biotechnology and Biological Sciences Research Council (grant no. BB/G016240/1 and funds from the Sustainable Energy Centre Cell Wall Sugars Programme).
dc.languageEnglishen
dc.language.isoenen
dc.publisherAmerican Society of Plant Biologists
dc.subjectPopulusen
dc.subjecthybrid aspenen
dc.subjectsecondary cell wallen
dc.subjecttertiary cell wallen
dc.subjectgelatinous fibersen
dc.subjecttension wooden
dc.subjectcontractile wallsen
dc.titleAspen Tension Wood Fibers Contain β-(1→4)-Galactans and Acidic Arabinogalactans Retained by Cellulose Microfibrils in Gelatinous Wallsen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from the American Society of Plant Biologists via http://dx.doi.org/10.​1104/​pp.​15.​00690en
prism.endingPage2063
prism.publicationDate2015en
prism.publicationNamePlant Physiologyen
prism.startingPage2048
prism.volume169en
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectidBB/G016240/1
dcterms.dateAccepted2015-09-12en
rioxxterms.versionofrecord10.1104/pp.15.00690en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2015-09-16en
dc.contributor.orcidTryfona, Theodora [0000-0002-1618-3521]
dc.contributor.orcidDupree, Paul [0000-0001-9270-6286]
dc.identifier.eissn1532-2548
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/G016240/1)


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