Show simple item record

dc.contributor.authorLeiwe, Marcus Nen
dc.contributor.authorHendry, Aenea Cen
dc.contributor.authorBard, Andrew Den
dc.contributor.authorEglen, Stephenen
dc.contributor.authorLowe, Andrew Sen
dc.contributor.authorThompson, Ian Den
dc.date.accessioned2016-01-13T11:20:41Z
dc.date.available2016-01-13T11:20:41Z
dc.date.issued2016-01-04en
dc.identifier.citationPLOS ONE 2016, 11(1): e0144846. doi:10.1371/journal.pone.0144846en
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253203
dc.description.abstractThe mouse dorsal lateral geniculate nucleus (dLGN) is an intermediary between retina and primary visual cortex (V1). Recent investigations are beginning to reveal regional complexity in mouse dLGN. Using local injections of retrograde tracers into V1 of adult and neonatal mice, we examined the developing organisation of geniculate projection columns: the population of dLGN-V1 projection neurons that converge in cortex. Serial sectioning of the dLGN enabled the distribution of labelled projection neurons to be reconstructed and collated within a common standardised space. This enabled us to determine: the organisation of cells within the dLGN-V1 projection columns; their internal organisation (topology); and their order relative to V1 (topography). Here, we report parameters of projection columns that are highly variable in young animals and refined in the adult, exhibiting profiles consistent with shell and core zones of the dLGN. Additionally, such profiles are disrupted in adult animals with reduced correlated spontaneous activity during development. Assessing the variability between groups with partial least squares regression suggests that 4–6 cryptic lamina may exist along the length of the projection column. Our findings further spotlight the diversity of the mouse dLGN–an increasingly important model system for understanding the pre-cortical organisation and processing of visual information. Furthermore, our approach of using standardised spaces and pooling information across many animals will enhance future functional studies of the dLGN.
dc.description.sponsorshipFunding was provided by a Wellcome Trust grant jointly awarded to IDT and SJE (083205, www.wellcome.ac.uk), and by MRC PhD Studentships awarded to MNL and ACH (http://www.mrc.ac.uk/).
dc.languageEnglishen
dc.language.isoenen
dc.publisherPLOS
dc.rightsAttribution 2.0 UK: England & Wales*
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/uk/*
dc.subjectNeuronsen
dc.subjectTopographic mapsen
dc.subjectMagnetic Resonance Imagingen
dc.subjectMiceen
dc.subjectHistologyen
dc.subjectRetinal ganglion cellsen
dc.subjectAnimal anatomyen
dc.subjectVisionen
dc.titleGeniculo-Cortical Projection Diversity Revealed within the Mouse Visual Thalamusen
dc.typeArticle
dc.description.versionThis is the final version of the article. It was first available from PLOS via http://dx.doi.org/10.1371/journal.pone.0144846en
prism.numbere0144846en
prism.publicationDate2016en
prism.publicationNamePLOS ONEen
prism.volume11en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderMRC
dc.rioxxterms.projectid083205
dcterms.dateAccepted2015-11-24en
rioxxterms.versionofrecord10.1371/journal.pone.0144846en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2016-01-04en
dc.contributor.orcidEglen, Stephen [0000-0001-8607-8025]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Reviewen


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 2.0 UK: England & Wales
Except where otherwise noted, this item's licence is described as Attribution 2.0 UK: England & Wales