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dc.contributor.authorTashiro, Yosukeen
dc.contributor.authorMonson, RItaen
dc.contributor.authorRamsay, Joshua Pen
dc.contributor.authorSalmond, Georgeen
dc.date.accessioned2016-01-13T11:31:23Z
dc.date.available2016-01-13T11:31:23Z
dc.date.issued2016-04-18en
dc.identifier.citationEnvironmental Microbiology 2016. doi:10.1111/1462-2920.13203en
dc.identifier.issn1462-2912
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/253204
dc.description.abstractDifferent modes of bacterial taxis play important roles in environmental adaptation, survival, colonization and dissemination of disease. One mode of taxis is flotation due to the production of gas vesicles. Gas vesicles are proteinaceous intracellular organelles, permeable only to gas, that enable flotation in aquatic niches. Gene clusters for gas vesicle biosynthesis are partially conserved in various archaea, cyanobacteria, and some proteobacteria, such as the enterobacterium, $\textit{Serratia sp.}$ ATCC 39006 (S39006). Here we present the first systematic analysis of the genes required to produce gas vesicles in S39006, identifying how this differs from the archaeon $\textit{Halobacterium salinarum}$. We define 11 proteins essential for gas vesicle production. Mutation of $\textit{gvpN}$ or $\textit{gvpV}$ produced small bicone gas vesicles, suggesting that the cognate proteins are involved in the morphogenetic assembly pathway from bicones to mature cylindrical forms. Using volumetric compression, gas vesicles were shown to comprise 17% of S39006 cells, whereas in $\textit{Escherichia coli}$ heterologously expressing the gas vesicle cluster in a deregulated environment, gas vesicles can occupy around half of cellular volume. Gas vesicle production in S39006 and $\textit{E. coli}$ was exploited to calculate the instantaneous turgor pressure within cultured bacterial cells; the first time this has been performed in either strain.
dc.description.sponsorshipThe authors would like to thank Professor Tony Walsby (Emeritus, Bristol university) for advice, technical help and donation of the pressure nephelometry and volumetric calculation apparatus. We would also like to thank Alison Drew for technical support and Chin Mei Lee and Andrew Day for critical reading. REM and GPCS were supported through the BBSRC (Grant ID BB/K001833/1). YT was supported by a Scientific Research Fellowship from the Japan Society for the Promotion of Sciences (JSPS) and JPR was supported by a Herschel Smith Post Doctoral Fellowship while at Cambridge University.
dc.languageEnglishen
dc.language.isoenen
dc.publisherWiley
dc.rightsAttribution 4.0 International
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleMolecular genetic and physical analysis of gas vesicles in buoyant enterobacteriaen
dc.typeArticle
dc.description.versionThis is the final version of the article. It first appeared from Wiley via https://doi.org/10.1111/1462-2920.13203en
prism.endingPage1276
prism.publicationDate2016en
prism.publicationNameEnvironmental Microbiologyen
prism.startingPage1264
prism.volume18en
dc.rioxxterms.funderBBSRC
dc.rioxxterms.projectidBB/K001833/1
dcterms.dateAccepted2015-12-29en
rioxxterms.versionofrecord10.1111/1462-2920.13203en
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-04-18en
dc.contributor.orcidSalmond, George [0000-0002-5197-2198]
dc.identifier.eissn1462-2920
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/K001833/1)
cam.orpheus.successThu Jan 30 12:55:26 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International