Research data supporting "Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis"

Nikolovski, Nino; Dupree, Ray; Brown, Steven P.; Dupree, Paul

dc.contributor.author	Nikolovski, Nino
dc.contributor.author	Dupree, Ray
dc.contributor.author	Brown, Steven P.
dc.contributor.author	Dupree, Paul
dc.date.accessioned	2016-03-23T16:08:22Z
dc.date.available	2016-03-23T16:08:22Z
dc.date.issued	2016-03-23
dc.identifier.uri	https://www.repository.cam.ac.uk/handle/1810/254646
dc.description	1D solid state NMR data for mature stems of stello cellulose synthesis mutant and wild type plants. MAS solid state NMR experiments were performed on a widebore Bruker (Karlsruhe, Germany) AVANCE III 850 MHz solid state NMR spectrometer operating at 20 T, corresponding to 1H and 13C Larmor frequencies of 850.2 and 213.8 MHz, respectively, using a 4 mm double-resonance MAS probe. Experiments were conducted at room temperature at a MAS frequency of 12.0 kHz ± 5 Hz. The 13C cross-polarization (CP) MAS spectra were acquired using a 1ms ramped contact time, a spectral window of 100 kHz (468 ppm), a recycle delay of 2 s and 28800 transients were co-added for each sample. The 1H 90° pulse duration was 3.4 s. Two-pulse phase-modulated (TPPM) decoupling was applied during acquisition at a 1H nutation frequency of 83 kHz58. The 13C chemical shift was determined using the carbonyl peak at 177.8 ppm of L-alanine as an external reference with respect to TMS. Two independent stem samples were analysed.	en
dc.description	This research data supports “Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis” which will be published in “Nature communications”.
dc.description.sponsorship	This work was supported by the BBSRC [grant number BB/G016240/1].	en
dc.format	zip, Word, docx
dc.publisher	University of Cambridge	en
dc.rights	Attribution 2.0 UK: England & Wales	*
dc.rights.uri	http://creativecommons.org/licenses/by/2.0/uk/	*
dc.subject	stello	en
dc.subject	solid state NMR	en
dc.subject	arabidopsis	en
dc.title	Research data supporting "Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis"	en
dc.type	Dataset	en
dc.rioxxterms.funder	BBSRC
dc.rioxxterms.projectid	BB/G016240/1
dc.identifier.doi	10.17863/CAM.68993
pubs.declined	2017-10-11T13:54:35.69+0100
datacite.contributor.supervisor	Dupree, Paul
dcterms.format	zip, docx
dc.contributor.orcid	Dupree, Paul [0000-0001-9270-6286]
datacite.issupplementto.doi	10.1038/ncomms11656