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dc.contributor.authorOtani, Tomokien
dc.contributor.authorMarchetto, Maria Cen
dc.contributor.authorGage, Fred Hen
dc.contributor.authorSimons, Benjaminen
dc.contributor.authorLivesey, Fredericken
dc.date.accessioned2016-04-07T09:21:04Z
dc.date.available2016-04-07T09:21:04Z
dc.date.issued2016-03-31en
dc.identifier.issn1934-5909
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/254848
dc.description.abstractVariation in cerebral cortex size and complexity is thought to contribute to differences in cognitive ability between humans and other animals. Here we compare cortical progenitor cell output in humans and three nonhuman primates using directed differentiation of pluripotent stem cells (PSCs) in adherent two-dimensional(2D)andorganoid three-dimensional (3D) culture systems. Clonal lineage analysis showed that primate cortical progenitors proliferate for a protracted period of time, during which they generate early-born neurons, in contrast to rodents, where this expansion phase largely ceases before neurogenesis begins. The extent of this additional cortical progenitor expansion differs among primates, leading to differences in the number of neurons generated by each progenitor cell. We found that this mechanism for controlling cortical size is regulated cell autonomously in culture, suggesting that primate cerebral cortex size is regulated at least in part at the level of individual cortical progenitor cell clonal output.
dc.description.sponsorshipT.O. was supported by the Wellcome Trust PhD Programme in Developmental Biology at the University of Cambridge. F.J.L. and B.D.S. are Wellcome Trust Investigators. This research was supported by core funding to the Gurdon Institute by the Wellcome Trust and Cancer Research UK. F.H.G. was supported by the Helmsley, Mathers, and JPB Foundations.
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.title2D and 3D stem cell models of primate cortical development identify species-specific differences in progenitor behavior contributing to brain sizeen
dc.typeArticle
dc.provenanceOA-7946
dc.description.versionThis is the final version of the article. It first appeared from Elsevier via https://doi.org/10.1016/j.stem.2016.03.003en
prism.endingPage480
prism.publicationDate2016en
prism.publicationNameCell Stem Cellen
prism.startingPage467
prism.volume18en
dc.rioxxterms.funderWellcome Trust
dc.rioxxterms.funderCRUK
dcterms.dateAccepted2016-03-10en
rioxxterms.versionofrecord10.1016/j.stem.2016.03.003en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2016-03-31en
dc.contributor.orcidSimons, Benjamin [0000-0002-3875-7071]
dc.contributor.orcidLivesey, Frederick [0000-0001-6128-3372]
dc.identifier.eissn1875-9777
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWELLCOME TRUST (098357/Z/12/Z)
pubs.funder-project-idMRC (MC_PC_12009)
cam.orpheus.successThu Jan 30 12:54:57 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International