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dc.contributor.authorImmanen, Juhaen
dc.contributor.authorNieminen, Kaisaen
dc.contributor.authorSmolander, Olli-Pekkaen
dc.contributor.authorKojima, Mikikoen
dc.contributor.authorSerra, Juan Alonsoen
dc.contributor.authorKoskinen, Patriken
dc.contributor.authorZhang, Jingen
dc.contributor.authorElo, Annakaisaen
dc.contributor.authorMähönen, Ari Pekkaen
dc.contributor.authorStreet, Nathanielen
dc.contributor.authorBhalerao, Rishikesh Pen
dc.contributor.authorPaulin, Larsen
dc.contributor.authorAuvinen, Petrien
dc.contributor.authorSakakibara, Hitoshien
dc.contributor.authorHelariutta, Yrjoen
dc.date.accessioned2016-06-23T09:17:34Z
dc.date.available2016-06-23T09:17:34Z
dc.date.issued2016-07-14en
dc.identifier.issn0960-9822
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/256447
dc.description.abstractDespite the crucial roles of phytohormones in plant development, comparison of the exact distribution profiles of different hormones within plant meristems has thus far remained scarce. Vascular cambium, a wide lateral meristem with an extensive developmental zonation, provides an optimal system for hormonal and genetic profiling. By taking advantage of this spatial resolution, we show here that two major phytohormones, cytokinin and auxin, display different yet partially overlapping distribution profiles across the cambium. In contrast to auxin, which has its highest concentration in the actively dividing cambial cells, cytokinins peak in the developing phloem tissue of a Populus trichocarpa stem. Gene expression patterns of cytokinin biosynthetic and signaling genes coincided with this hormonal gradient. To explore the functional significance of cytokinin signaling for cambial development, we engineered transgenic Populus tremula × tremuloides trees with an elevated cytokinin biosynthesis level. Confirming that cytokinins function as major regulators of cambial activity, these trees displayed stimulated cambial cell division activity resulting in dramatically increased (up to 80% in dry weight) production of the lignocellulosic trunk biomass. To connect the increased growth to hormonal status, we analyzed the hormone distribution and genome-wide gene expression profiles in unprecedentedly high resolution across the cambial zone. Interestingly, in addition to showing an elevated cambial cytokinin content and signaling level, the cambial auxin concentration and auxin-responsive gene expression were also increased in the transgenic trees. Our results indicate that cytokinin signaling specifies meristematic activity through a graded distribution that influences the amplitude of the cambial auxin gradient.
dc.description.sponsorshipJ.I., K.N., J.A.S. and Y.H. were funded by ERC, Fibic EffFibre, Academy of Finland (by Centre of Excellence and other programs) and Tekes. O.P.S., L.P. and P.A. were funded by Academy of Finland. The hormone analysis was supported by Japan Advanced Plant Science Network. R.P.B. was funded by grants from Berzili, TC4F and FUTURE trees.
dc.languageEnglishen
dc.language.isoenen
dc.publisherElsevier (Cell Press)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleCytokinin and auxin display distinct but interconnected distribution and signaling profiles to stimulate cambial activityen
dc.typeArticle
dc.description.versionThis is the author accepted manuscript. The final version is available from Cell Press via http://dx.doi.org/10.1016/j.cub.2016.05.053en
prism.publicationDate2016en
prism.publicationNameCurrent Biologyen
dc.identifier.doi10.17863/CAM.391
dcterms.dateAccepted2016-05-23en
rioxxterms.versionofrecord10.1016/j.cub.2016.05.053en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en
rioxxterms.licenseref.startdate2016-07-14en
dc.contributor.orcidHelariutta, Yrjo [0000-0002-7287-8459]
dc.identifier.eissn1879-0445
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Research Council (323052)
rioxxterms.freetoread.startdate2017-07-14


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International