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dc.contributor.authorCampbell, Jonathanen
dc.contributor.authorHusmann, Ankeen
dc.contributor.authorHume, Roberten
dc.contributor.authorWatson, Christineen
dc.contributor.authorCameron, Ruthen
dc.date.accessioned2017-01-25T10:00:23Z
dc.date.available2017-01-25T10:00:23Z
dc.date.issued2017-01-01en
dc.identifier.issn0142-9612
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/262024
dc.description.abstractCancer is characterized by cell heterogeneity and the development of 3D $\textit{in vitro }$ assays that can distinguish more invasive or migratory phenotypes could enhance diagnosis or drug discovery. 3D collagen scaffolds have been used to develop analogues of complex tissues $\textit{in vitro }$ and are suited to routine biochemical and immunological assays. We sought to increase 3D model tractability and modulate the migration rate of seeded cells using an ice-templating technique to create either directional/anisotropic or non-directional/isotropic porous architectures within cross-linked collagen scaffolds. Anisotropic scaffolds supported the enhanced migration of an invasive breast cancer cell line MDA-MB-231 with an altered spatial distribution of proliferative cells in contrast to invasive MDA-MB-468 and non-invasive MCF-7 cells lines. In addition, MDA-MB-468 showed increased migration upon epithelial-to-mesenchymal transition (EMT) in anisotropic scaffolds. The provision of controlled architecture in this system may act both to increase assay robustness and as a tuneable parameter to capture detection of a migrated population within a set time, with consequences for primary tumour migration analysis. The separation of invasive clones from a cancer biomass with in vitro platforms could enhance drug development and diagnosis testing by contributing assay metrics including migration rate, as well as modelling cell-cell and cell-matrix interaction in a system compatible with routine histopathological testing.
dc.description.sponsorshipThe authors gratefully acknowledge the financial support of the ERC Advanced Grant 320598 3D-E and the Newton Trust. A.H. held a Daphne Jackson Fellowship funded by the University of Cambridge for part of the work. R.D.H. is funded through a NC3Rs studentship.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectbreast canceren
dc.subjectthree dimensional migrationen
dc.subjectcollagen 1en
dc.subjectice-templating techniqueen
dc.subjectscaffold architectureen
dc.subjectinvasionen
dc.titleDevelopment of three-dimensional collagen scaffolds with controlled architecture for cell migration studies using breast cancer cell linesen
dc.typeArticle
prism.endingPage43
prism.publicationDate2017en
prism.publicationNameBiomaterialsen
prism.startingPage34
prism.volume114en
dc.identifier.doi10.17863/CAM.7262
dcterms.dateAccepted2016-10-28en
rioxxterms.versionofrecord10.1016/j.biomaterials.2016.10.048en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-01-01en
dc.contributor.orcidHusmann, Anke [0000-0001-5326-3785]
dc.contributor.orcidWatson, Christine [0000-0002-8548-5902]
dc.contributor.orcidCameron, Ruth [0000-0003-1573-4923]
dc.identifier.eissn1878-5905
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idNC3Rs (NC/K001655/1)
pubs.funder-project-idMRC (MR/K011014/1)
pubs.funder-project-idCancer Research Institute (CRI) (unknown)
pubs.funder-project-idBBSRC (BB/H006206/1)
pubs.funder-project-idEuropean Research Council (320598)
cam.issuedOnline2016-11-01en
cam.orpheus.successThu Jan 30 12:53:52 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International