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dc.contributor.authorde, Vries SPWen
dc.contributor.authorBaig, Aen
dc.contributor.authorCoward, Cen
dc.contributor.authorMaskell, Duncanen
dc.contributor.authorGrant, Andrewen
dc.date.accessioned2017-02-24T15:22:07Z
dc.date.available2017-02-24T15:22:07Z
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/262774
dc.description.abstractTo investigate how $\textit{Campylobacter jejuni}$ causes the clinical symptoms of diarrhoeal disease in humans, use of a relevant animal model is essential. Such a model should mimic the human disease closely in terms of host physiology, incubation period before onset of disease, clinical signs and a comparable outcome of disease. In this study, we used a gnotobiotic piglet model to study determinants of pathogenicity of C. $\textit{C. jejuni}$. In this model, C. $\textit{C. jejuni}$ successfully established infection and piglets developed an increased temperature with watery diarrhoea, which was caused by a leaky epithelium and reduced bile re-absorption in the intestines. Further, we assessed the C. $\textit{C. jejuni}$ genes required for infection of the porcine gastrointestinal tract utilising a transposon (Tn) mutant library screen. A total of 123 genes of which Tn mutants showed attenuated piglet infection were identified. Our screen highlighted a crucial role for motility and chemotaxis, as well as central metabolism. In addition, Tn mutants of 14 genes displayed enhanced piglet infection. This study gives a unique insight into the mechanisms of C. $\textit{C. jejuni}$ disease in terms of host physiology and contributing bacterial factors.
dc.description.sponsorshipThis work was funded by BBSRC awards BB/F020988/1 and BB/K004514/1. Moredun Research Institute receives funding via Rural and Environment Science and Analytical Services (RESAS) division of the Scottish Government.
dc.languageEnglishen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.titleAnalysis of $\textit{Campylobacter jejuni}$ infection in the gnotobiotic piglet and genome-wideen
dc.typeArticle
prism.publicationNameScientific Reportsen
dc.identifier.doi10.17863/CAM.8064
dcterms.dateAccepted2017-02-06en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-02-06en
dc.contributor.orcidMaskell, Duncan [0000-0002-5065-653X]
dc.contributor.orcidGrant, Andrew [0000-0001-9746-2989]
rioxxterms.typeJournal Article/Reviewen
cam.orpheus.successThu Jan 30 12:56:36 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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