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dc.contributor.authorMiller, SRen
dc.contributor.authorPerera, Surangien
dc.contributor.authorBaker, Clareen
dc.date.accessioned2017-04-06T13:49:48Z
dc.date.available2017-04-06T13:49:48Z
dc.date.issued2017-03-15en
dc.identifier.issn2046-6390
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/263487
dc.description.abstractPerivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1 activation (via expression of the Notch1 intracellular domain) is sufficient $\textit{in vivo}$ to convert trunk mesoderm-derived somite cells to perivascular cells, at the expense of skeletal muscle. In experiments originally designed to investigate the effect of premature Notch1 activation on the development of neural crest-derived olfactory ensheathing glial cells (OECs), we used $\textit{in ovo}$ electroporation to insert a tetracycline-inducible $\textit{NotchΔE}$ construct (encoding a constitutively active mutant of mouse Notch1) into the genome of chicken cranial neural crest cell precursors, and activated $\textit{NotchΔE}$ expression by doxycycline injection at embryonic day 4. $\textit{NotchΔE}$-targeted cells formed perivascular cells within the frontonasal mesenchyme, and expressed a perivascular marker on the olfactory nerve. Hence, constitutively activating Notch1 is sufficient $\textit{in vivo}$ to drive not only somite cells, but also neural crest-derived frontonasal mesenchyme and perhaps developing OECs, to a perivascular cell fate. These results also highlight the plasticity of neural crest-derived mesenchyme and glia.
dc.description.sponsorshipS.R.M. was supported by a PhD research studentship from the Anatomical Society, with additional funding from the Cambridge Philosophical Society. S.N.P. was supported by the Wellcome Trust (PhD Studentship 102453/Z/13/Z) and the Cambridge Commonwealth Trust.
dc.languageengen
dc.language.isoenen
dc.publisherCompany of Biologists
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.subjectchick embryoen
dc.subjectfrontonasal mesenchymeen
dc.subjectneural cresten
dc.subjectnotchen
dc.subjectolfactory ensheathing cellsen
dc.subjectpericyteen
dc.titleConstitutively active Notch1 converts cranial neural crest-derived frontonasal mesenchyme to perivascular cells $\textit{in vivo}$en
dc.typeArticle
prism.endingPage325
prism.issueIdentifier3en
prism.publicationDate2017en
prism.publicationNameBiology Openen
prism.startingPage317
prism.volume6en
dc.identifier.doi10.17863/CAM.8832
dcterms.dateAccepted2017-01-20en
rioxxterms.versionofrecord10.1242/bio.023887en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-03-15en
dc.contributor.orcidBaker, Clare [0000-0002-4434-3107]
dc.identifier.eissn2046-6390
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (102453/Z/13/Z)
pubs.funder-project-idAnatomical Society (AS) (30058)
cam.issuedOnline2017-02-09en
cam.orpheus.successThu Jan 30 12:56:34 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International