Show simple item record

dc.contributor.authorMair, Pen
dc.contributor.authorGielen, Fen
dc.contributor.authorHollfelder, Florianen
dc.date.accessioned2017-05-05T12:21:12Z
dc.date.available2017-05-05T12:21:12Z
dc.date.issued2017-04-05en
dc.identifier.issn1367-5931
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/264099
dc.description.abstractScreening of enzyme mutants in monodisperse picoliter compartments, generated at kilohertz speed in microfluidic devices, is coming of age. After a decade of proof-of-principle experiments, workflows have emerged that combine existing microfluidic modules to assay reaction progress quantitatively and yield improved enzymes. Recent examples of the screening of libraries of randomised proteins and from metagenomic sources suggest that this approach is not only faster and cheaper, but solves problems beyond the feasibility scope of current methodologies. The establishment of new assays in this format – so far covering hydrolases, aldolases, polymerases and dehydrogenases – will enable the exploration of sequence space for new catalysts of natural and non-natural chemical transformations.
dc.description.sponsorshipThis work was funded by the Engineering and Physical Sciences Research Council (EPSRC; studentship in the Centre for Doctoral Training “Sensor Technologies and Applications” to P.M., EP/L015889/1) and the Biotechnology and Biological Research Council (BBSRC; BB/K013629/1). FH is an ERC Advanced Investigator.
dc.languageengen
dc.language.isoenen
dc.publisherElsevier
dc.titleExploring sequence space in search of functional enzymes using microfluidic dropletsen
dc.typeArticle
prism.endingPage144
prism.publicationDate2017en
prism.publicationNameCurrent Opinion in Chemical Biologyen
prism.startingPage137
prism.volume37en
dc.identifier.doi10.17863/CAM.9462
dcterms.dateAccepted2017-02-15en
rioxxterms.versionofrecord10.1016/j.cbpa.2017.02.018en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-04-05en
dc.contributor.orcidHollfelder, Florian [0000-0002-1367-6312]
dc.identifier.eissn1879-0402
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/K013629/1)
pubs.funder-project-idEPSRC (EP/L015889/1)
rioxxterms.freetoread.startdate2018-04-05


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record