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dc.contributor.authorZeineddine, Ren
dc.contributor.authorWhiten, Danielen
dc.contributor.authorFarrawell, NEen
dc.contributor.authorMcAlary, Len
dc.contributor.authorHanspal, Mayaen
dc.contributor.authorKumita, Janeten
dc.contributor.authorWilson, MRen
dc.contributor.authorYerbury, JJen
dc.date.accessioned2017-07-06T11:27:24Z
dc.date.available2017-07-06T11:27:24Z
dc.date.issued2017-05-09en
dc.identifier.issn1933-6896
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/265201
dc.description.abstractAmyotrophic lateral sclerosis is a devastating neuromuscular degenerative disease characterized by a focal onset of motor neuron loss, followed by contiguous outward spreading of pathology including TAR DNA-binding protein of 43 kDa (TDP-43) aggregates. Previous work suggests that TDP-43 can move between cells. Here we used a novel flow cytometry technique (FloIT) to analyze TDP-43 inclusions and propagation. When cells were transfected to express either mutant G294A TDP-43 fused to GFP or wild type TDP-43fused to tomato red and then co-cultured, flow cytometry detected intact cells containing both fusion proteins and using FloIT detected an increase in the numbers of inclusions in lysates from cells expressing wild type TDP-43-tomato. Furthermore, in this same model, FloIT analyses detected inclusions containing both fusion proteins. These results imply the transfer of TDP-43 fusion proteins between cells and that this process can increase aggregation of wild-type TDP-43 by a mechanism involving co-aggregation with G294A TDP-43.
dc.description.sponsorshipThis work was supported by the NHMRC under Grant 1084144 and 1095215.
dc.languageengen
dc.language.isoenen
dc.publisherTaylor & Francis
dc.subjectALSen
dc.subjectFloITen
dc.subjectFlow cytometryen
dc.subjectPrionen
dc.subjectpropagationen
dc.subjectprotein aggregationen
dc.subjectTDP-43en
dc.titleFlow cytometric measurement of the cellular propagation of TDP-43 aggregationen
dc.typeArticle
prism.endingPage204
prism.issueIdentifier3en
prism.publicationDate2017en
prism.publicationNamePrionen
prism.startingPage195
prism.volume11en
dc.identifier.doi10.17863/CAM.11257
dcterms.dateAccepted2017-03-28en
rioxxterms.versionofrecord10.1080/19336896.2017.1314426en
rioxxterms.versionAMen
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-05-09en
dc.contributor.orcidWhiten, Daniel [0000-0002-7853-3566]
dc.contributor.orcidKumita, Janet [0000-0002-3887-4964]
dc.identifier.eissn1933-690X
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2017-05-09en
rioxxterms.freetoread.startdate2018-05-09


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