Show simple item record

dc.contributor.authorLudden, Catherineen
dc.contributor.authorReuter, Sen
dc.contributor.authorJudge, Ken
dc.contributor.authorGouliouris, Ten
dc.contributor.authorBlane, Ben
dc.contributor.authorColl, Fen
dc.contributor.authorNaydenova, Plamenaen
dc.contributor.authorHunt, Men
dc.contributor.authorTracey, Aen
dc.contributor.authorHopkins, Ken
dc.contributor.authorBrown, Nen
dc.contributor.authorWoodford, Nen
dc.contributor.authorParkhill, Julianen
dc.contributor.authorPeacock, Sharonen
dc.date.accessioned2017-07-13T11:54:06Z
dc.date.available2017-07-13T11:54:06Z
dc.date.issued2017-07-04en
dc.identifier.issn2057-5858
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/265284
dc.description.abstractDissemination of carbapenem resistance among pathogenic Gram-negative bacteria is a looming medical emergency. Efficient spread of resistance within and between bacterial species is facilitated by mobile genetic elements. We hypothesized that wastewater contributes to the dissemination of carbapenemase-producing Enterobacteriaceae (CPE), and studied this through a cross-sectional observational study of wastewater in the East of England. We isolated clinically relevant species of CPE in untreated and treated wastewater, confirming that waste treatment does not prevent release of CPE into the environment. We observed that CPE-positive plants were restricted to those in direct receipt of hospital waste, suggesting that hospital effluent may play a role in disseminating carbapenem resistance. We postulated that plasmids carrying carbapenemase genes were exchanged between bacterial hosts in sewage, and used short-read (Illumina) and long-read (MinION) technologies to characterize plasmids encoding resistance to antimicrobials and heavy metals. We demonstrated that different CPE species ($\textit{Enterobacter kobei}$ and $\textit{Raoultella ornithinolytica}$) isolated from wastewater from the same treatment plant shared two plasmids of 63 and 280 kb. The former plasmid conferred resistance to carbapenems ($bla_\text{OXA-48}$), and the latter to numerous drug classes and heavy metals. We also report the complete genome sequence for $\textit{Enterobacter kobei}$. Small, portable sequencing instruments such as the MinION have the potential to improve the quality of information gathered on antimicrobial resistance in the environment.
dc.description.sponsorshipThis publication presents independent research supported by the Health Innovation Challenge Fund (WT098600, HICF-T5-342), a parallel funding partnership between the Department of Health, UK, and the Wellcome Trust. C. L. is a Wellcome Trust Sir Henry Postdoctoral Fellow (110243/Z/15/Z). T. G. is a Wellcome Trust Research Training Fellow (103387/Z/13/Z).
dc.language.isoenen
dc.publisherMicrobiology Society
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleSharing of carbapenemase-encoding plasmids between Enterobacteriaceae in UK sewage uncovered by MinION sequencingen
dc.typeArticle
prism.publicationDate2017en
prism.publicationNameMicrobial genomicsen
dc.identifier.doi10.17863/CAM.11290
dcterms.dateAccepted2017-04-13en
rioxxterms.versionofrecord10.1099/mgen.0.000114en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-07-04en
dc.contributor.orcidLudden, Catherine [0000-0001-9503-0744]
dc.contributor.orcidParkhill, Julian [0000-0002-7069-5958]
dc.contributor.orcidPeacock, Sharon [0000-0002-1718-2782]
dc.identifier.eissn2057-5858
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (098600/Z/12/Z)
pubs.funder-project-idWellcome Trust (103387/Z/13/Z)
cam.issuedOnline2017-07-04en
cam.orpheus.successThu Jan 30 12:53:44 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International