Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules
Palacios De Castro, Isabel
Nature Publishing Group
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Drechsler, M., Giavazzi, F., Cerbino, R., & Palacios De Castro, I. (2017). Active diffusion and advection in Drosophila oocytes result from the interplay of actin and microtubules. Nature Communications, 8 (1520)https://doi.org/10.1038/s41467-017-01414-6
Transport in cells occurs via a delicate interplay of passive and active processes, including diffusion, directed transport and advection. Despite progress in super-resolution microscopy, discriminating and quantifying these processes is a challenge, requiring tracking of rapidly moving, sub-diffraction objects in a crowded, noisy environment. Here we use Differential Dynamic Microscopy with different contrast mechanisms to provide a thorough characterization of the dynamics in the Drosophila oocyte. We study the movement of vesicles and the elusive motion of a cytoplasmic F-actin mesh, a known regulator of cytoplasmic flows. We find that cytoplasmic motility constitutes a combination of directed motion and random diffusion. While advection is mainly attributed to microtubules, we find that active diffusion is driven by the actin cytoskeleton, although it is also enhanced by the flow. We also find that an important dynamic link exists between vesicles and cytoplasmic F-actin motion, as recently suggested in mouse oocytes.
self-organization, active processes, motion, body plan, oogenesis, cell asymmetries, cytoskeleton, microtubules, actin, motor proteins
MD and IMP were supported by the BBSRC, the Department of Zoology (Cambridge), the University of Cambridge, and an Isaac Newton Trust fellowship to MD. FG and RC acknowledge funding by the Italian Ministry of Education and Research, Futuro in Ricerca Project ANISOFT (RBFR125H0M) and by Fondazione CARIPLO-Regione Lombardia Project Light for Life (2016-0998).
External DOI: https://doi.org/10.1038/s41467-017-01414-6
This record's URL: https://www.repository.cam.ac.uk/handle/1810/267869
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/