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dc.contributor.authorMcQuitty, Ruth J
dc.contributor.authorUnterkofler, Sarah
dc.contributor.authorEuser, Tijmen G
dc.contributor.authorRussell, Philip St J
dc.contributor.authorSadler, Peter J
dc.date.accessioned2017-12-11T12:59:54Z
dc.date.available2017-12-11T12:59:54Z
dc.date.issued2017-07-29
dc.identifier.issn2046-2069
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/270123
dc.description.abstractWe explore the efficacy of a hyphenated photonic crystal fibre microflow reactor - high-resolution mass spectrometer system as a method for screening the activity of potential new photoactivatable drugs. The use of light to activate drugs is an area of current development as it offers the possibility of reduced side effects due to improved spatial and temporal targeting and novel mechanisms of anticancer activity. The di-nuclear ruthenium complex [{(η6-indan)RuCl}2(μ-2,3-dpp)](PF6)2, previously studied by Magennis et al. (Inorg. Chem., 2007, 46, 5059) is used as a model drug to compare the system to standard irradiation techniques. The photodecomposition pathways using blue light radiation are the same for PCF and conventional cuvette methods. Reactions in the presence of small biomolecules 5'-guanosine monophosphate (5'-GMP), 5'-adenosine monophosphate (5'-AMP), l-cysteine (l-Cys) and glutathione (γ-l-glutamyl-l-cysteinyl-glycine, GSH) were studied. The complex was found to bind to nucleobases in the dark and this binding increased upon irradiation with 488 nm light, forming the adducts [(η6-indan)Ru2(μ-2,3-dpp) + 5'-GMP]2+ and [(η6-indan)Ru + (5'-AMP)]+. These findings are consistent with studies using conventional methods. The dinuclear complex also binds strongly to GSH after irradiation, a possible explanation for its lack of potency in cell line testing. The use of the PCF-MS system dramatically reduced the sample volume required and reduced the irradiation time by four orders of magnitude from 14 hours to 12 seconds. However, the reduced sample volume also results in a reduced MS signal intensity. The dead time of the combined system is 15 min, limited by the intrinsic dead volume of the HR-MS.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherRoyal Society of Chemistry (RSC)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleRapid screening of photoactivatable metallodrugs: photonic crystal fibre microflow reactor coupled to ESI mass spectrometry.
dc.typeArticle
prism.endingPage37348
prism.issueIdentifier59
prism.publicationDate2017
prism.publicationNameRSC Adv
prism.startingPage37340
prism.volume7
dc.identifier.doi10.17863/CAM.16940
dcterms.dateAccepted2017-07-17
rioxxterms.versionofrecord10.1039/c7ra06735f
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2017-07-26
dc.contributor.orcidMcQuitty, Ruth J [0000-0003-0632-6661]
dc.contributor.orcidUnterkofler, Sarah [0000-0002-9748-646X]
dc.contributor.orcidEuser, Tijmen G [0000-0002-8305-9598]
dc.contributor.orcidRussell, Philip St J [0000-0002-8972-2477]
dc.contributor.orcidSadler, Peter J [0000-0001-9160-1941]
dc.identifier.eissn2046-2069
rioxxterms.typeJournal Article/Review
cam.issuedOnline2017-07-26


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International