Variability of human pluripotent stem cell lines.
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Publication Date
2017-10Journal Title
Curr Opin Genet Dev
ISSN
0959-437X
Publisher
Elsevier BV
Volume
46
Pages
179-185
Language
eng
Type
Article
This Version
AM
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Ortmann, D., & Vallier, L. (2017). Variability of human pluripotent stem cell lines.. Curr Opin Genet Dev, 46 179-185. https://doi.org/10.1016/j.gde.2017.07.004
Abstract
Human pluripotent stem cells derived from embryos (human Embryonic Stem Cells or hESCs) or generated by direct reprogramming of somatic cells (human Induced Pluripotent Stem Cells or hiPSCs) can proliferate almost indefinitely in vitro while maintaining the capacity to differentiate into a broad diversity of cell types. These two properties (self-renewal and pluripotency) confers human pluripotent stem cells a unique interest for clinical applications since they could allow the production of infinite quantities of cells for disease modelling, drug screening and cell based therapy. However, recent studies have clearly established that human pluripotent stem cell lines can display variable capacity to differentiate into specific lineages. Consequently, the development of universal protocols of differentiation which could work efficiently with any human pluripotent cell line is complicated substantially. As a consequence, each protocol needs to be adapted to every cell line thereby limiting large scale applications and precluding personalised therapies. Here, we summarise our knowledge concerning the origin of this variability and describe potential solutions currently available to bypass this major challenge.
Keywords
Cell Line, Humans, Cell Differentiation, Embryonic Stem Cells, Induced Pluripotent Stem Cells, Cellular Reprogramming
Sponsorship
Medical Research Council (MC_PC_12009)
Identifiers
External DOI: https://doi.org/10.1016/j.gde.2017.07.004
This record's URL: https://www.repository.cam.ac.uk/handle/1810/270151
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