The Medicago truncatula GRAS protein RAD1 supports arbuscular mycorrhiza symbiosis and Phytophthora palmivora susceptibility.
Authors
Bonhomme, Maxime
Chatterjee, Abhishek
Toulotte, Justine
André, Olivier
Publication Date
2017-12Journal Title
Journal of experimental botany
ISSN
0022-0957
Publisher
OUP
Volume
68
Issue
21-22
Pages
5871-5881
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
Show full item recordCitation
Rey, T., Bonhomme, M., Chatterjee, A., Gavrin, A., Toulotte, J., Yang, W., André, O., et al. (2017). The Medicago truncatula GRAS protein RAD1 supports arbuscular mycorrhiza symbiosis and Phytophthora palmivora susceptibility.. Journal of experimental botany, 68 (21-22), 5871-5881. https://doi.org/10.1093/jxb/erx398
Abstract
The roots of most land plants are colonised by symbiotic arbuscular mycorrhiza (AM) fungi. To facilitate this symbiosis, plant genomes encode a set of genes required for microbial perception and accommodation. However, the extent to which infection by filamentous root pathogens also relies on some of these genes remains an open question. Here, we used genome-wide association mapping to identify genes contributing to colonisation of Medicago truncatula roots by the pathogenic oomycete Phytophthora palmivora. Single-nucleotide polymorphism (SNP) markers most significantly associated with plant colonisation response were identified upstream of RAD1, which encodes a GRAS transcription regulator first negatively implicated in root nodule symbiosis and recently identified as a positive regulator of AM symbiosis. RAD1 transcript levels are upregulated both in response to AM fungus and to a lower extent in infected tissues by P. palmivora where its expression is restricted to root cortex cells proximal to pathogen hyphae. Reverse genetics showed that reduction of RAD1 transcript levels as well as a rad1 mutant are impaired in their full colonisation by AM fungi as well as by P. palmivora. Thus, the importance of RAD1 extends beyond symbiotic interactions, suggesting a general involvement in M. truncatula microbe-induced root development and interactions with unrelated beneficial and detrimental filamentous microbes.
Keywords
Phytophthora, Mycorrhizae, Medicago truncatula, Disease Susceptibility, Endodeoxyribonucleases, Plant Proteins, Symbiosis, Plant Diseases, Gene Expression Regulation, Plant, Host-Pathogen Interactions, Genome-Wide Association Study
Sponsorship
Royal Society (uf110073)
Gatsby Charitable Foundation (unknown)
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (637537)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1093/jxb/erx398
This record's URL: https://www.repository.cam.ac.uk/handle/1810/271152
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