Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development.
Nahorski, Michael Stefan
Shaikh, Samiha S
Nature Publishing Group
MetadataShow full item record
Nahorski, M. S., Borner, G. H., Shaikh, S. S., Davies, A., Al-Gazali, L., Antrobus, R., & Woods, G. (2018). Clathrin heavy chain 22 contributes to the control of neuropeptide degradation and secretion during neuronal development.. Scientific reports, 8 (1), 2340. https://doi.org/10.1038/s41598-018-19980-0
The repertoire of cell types in the human nervous system arises through a highly orchestrated process, the complexity of which is still being discovered. Here, we present evidence that CHC22 has a non-redundant role in an early stage of neural precursor differentiation, providing a potential explanation of why CHC22 deficient patients are unable to feel touch or pain. We show the CHC22 effect on neural differentiation is independent of the more common clathrin heavy chain CHC17, and that CHC22-dependent differentiation is mediated through an autocrine/paracrine mechanism. Using quantitative proteomics, we define the composition of clathrin-coated vesicles in SH-SY5Y cells, and determine proteome changes induced by CHC22 depletion. In the absence of CHC22 a subset of dense core granule (DCG) neuropeptides accumulated, were processed into biologically active ‘mature’ forms, and secreted in sufficient quantity to trigger neural differentiation. When CHC22 is present, however, these DCG neuropeptides are directed to the lysosome and degraded, thus preventing differentiation. This suggests that the brief reduction seen in CHC22 expression in sensory neural precursors may license a step in neuron precursor neurodevelopment; and that this step is mediated through control of a novel neuropeptide processing pathway.
Neurons, Cell Line, Tumor, Lysosomes, Humans, Neuropeptides, Clathrin Heavy Chains, Autocrine Communication, Paracrine Communication, Cell Differentiation, Protein Transport, Gene Knockdown Techniques, Proteolysis
Cambridge University Hospitals NHS Foundation Trust (CUH) (3819-1415-50)
Embargo Lift Date
External DOI: https://doi.org/10.1038/s41598-018-19980-0
This record's URL: https://www.repository.cam.ac.uk/handle/1810/273997
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
Recommended or similar items
The following licence files are associated with this item: