No metabolic effects of mustard allyl-isothiocyanate compared with placebo in men.
Tan, Chong Yew
Soeters, Maarten R
Watson, Laura Pe
Murgatroyd, Peter R
The American journal of clinical nutrition
American Society for Clinical Nutrition, Inc.
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Langeveld, M., Tan, C. Y., Soeters, M. R., Virtue, S., Watson, L. P., Murgatroyd, P. R., Ambler, G. K., et al. (2017). No metabolic effects of mustard allyl-isothiocyanate compared with placebo in men.. The American journal of clinical nutrition, 106 (5), 1197-1205. https://doi.org/10.3945/ajcn.116.148395
ABSTRACT Background: Induction of nonshivering thermogenesis can be used to influence energy balance to prevent or even treat obesity. The pungent component of mustard, allyl-isothiocyanate (AITC), activates the extreme ½AQ3 cold receptor transient receptor potential channel, subfamily A, member 1 and may thus induce energy expenditure and metabolic changes. Objective: The objective of our study was to evaluate the potential of mustard AITC to induce thermogenesis (primary outcome) and alter body temperature, cold and hunger sensations, plasma metabolic parameters, and energy intake (secondary outcomes). Design: Energy expenditure in mice was measured after subcutane½ AQ4 ous injection with vehicle, 1 mg norepinephrine/kg, ½AQ5 or 5 mg AITC/kg. In our human crossover study, 11 healthy subjects were studied under temperature-controlled conditions after an overnight fast. After ingestion of 10 g of capsulated mustard or uncapsulated ½AQ6 mustard or a capsulated placebo mixture, measurements of energy expenditure, substrate oxidation, core temperature, cold and hunger scores, and plasma parameters were repeated every 30 min during a 150-min period. Subjects were randomly selected for the placebo and capsulated mustard intervention; 9 of 11 subjects received the uncapsulated mustard as the final intervention because this could not be blinded. After the experiments, energy intake was measured with the universal eating monitor in a test meal. Results: In mice, AITC administration induced a 32% increase in energy expenditure compared with placebo (17.5 6 4.9 J $ min21 $ mouse21 compared with 12.5 6 1.2 J $ min21 $ mouse21, P = 0.03). Of the 11 randomly selected participants, 1 was excluded because of intercurrent illness after the first visit and 1 withdrew after the second visit. Energy expenditure did not increase after ingestion of capsulated or uncapsulated mustard compared with placebo. No differences in substrate oxidation, core temperature, cold and hunger scores, or plasma parameters were found, nor was the energy intake at the end of the experiment different between the 3 conditions. Conclusion: The highest tolerable dose of mustard we were able to use did not elicit a relevant thermogenic response in humans. This trial was registered at www.controlled-trials.com as ISRCTN19147515. Am J Clin Nutr 2017;106:1–9.
Animals, Humans, Mice, Mustard Plant, Norepinephrine, Isothiocyanates, Blood Glucose, Body Temperature, Cross-Over Studies, Hunger, Energy Metabolism, Oxygen Consumption, Energy Intake, Thermogenesis, Adolescent, Adult, Aged, Middle Aged, Female, Male, Young Adult
Wellcome Trust (095564/Z/11/Z)
Department of Health (via National Institute for Health Research (NIHR)) (NF-SI-0514-10176)
Biotechnology and Biological Sciences Research Council (BB/J009865/1)
Medical Research Council (MC_UU_12012/2)
Medical Research Council (MC_UU_12012/5)
Embargo Lift Date
External DOI: https://doi.org/10.3945/ajcn.116.148395
This record's URL: https://www.repository.cam.ac.uk/handle/1810/274787
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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