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dc.contributor.authorLuthman, Anna Siri Naemi
dc.date.accessioned2018-04-16T16:38:46Z
dc.date.available2018-04-16T16:38:46Z
dc.date.issued2018-07-21
dc.date.submitted2017-09-27
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/274904
dc.description.abstractThe ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyper and multispectral imaging facilitate the detection of both spatial and spectral information in a single data acquisition, however, instrumentation for spatiospectral data acquisition is typically complex, bulky and expensive. This thesis seeks to overcome these limitations by using recently commercialised compact and robust hyper/multispectral cameras based on spectrally resolved detector arrays. Following sensor calibrations, which devoted particular attention to the angular sensitivity of the sensors, we integrated spectrally resolved detector arrays into a wide-field and an endoscopic imaging platform. This allowed multiplexed reflectance and fluorescence imaging with spectrally resolved detector array technology in vitro, in tissue mimicking phantoms, in an ex vivo oesophageal model and in vivo in a mouse model. A hyperspectral linescan sensor was first integrated in a wide-field near-infrared reflectance based imaging set-up to assess the suitability of spectrally resolved detector arrays for in vivo imaging of exogenous fluorescent contrast agents. Using this fluorescence hyperspectral imaging system, we could accurately resolve the presence and concentration of seven fluorescent dyes in solution. We also demonstrated high spectral unmixing precision, signal linearity with dye concentration, at depth in tissue mimicking phantoms, and delineation of four fluorescent dyes in vivo. After the successful demonstration of multiplexed fluorescence imaging in a wide-field set-up, we proceeded to combine near-infrared multiplexed fluorescence imaging with visible light spectral reflectance imaging in an endoscopic set-up. A multispectral endoscopic imaging system, capable of simultaneous reflectance and fluorescence imaging, was developed around two snapshot spectrally resolved detector arrays. In the process of system integration and characterisation, methods to characterise and predict the imaging performance of spectral endoscopes were developed. With the endoscope we demonstrated simultaneous imaging and spectral unmixing of chemically oxy/deoxygenated blood and three fluorescent dyes in a tissue mimicking phantom, and of two fluorescent dyes in an ex vivo oesophageal porcine model. With further developments, this technology has the potential to become applicable in medical imaging for detection of diseases such as gastrointestinal cancers.
dc.description.sponsorshipFunding was obtained from the Schiff Foundation and the Foundation Blanceflor
dc.language.isoen
dc.rightsAll rights reserved
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.subjecthyperspectral
dc.subjectbiomedical
dc.subjectin vivo
dc.subjectmultiplexed
dc.subjectfluorescence
dc.subjectSRDA
dc.subjectCMOS
dc.titleSpectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging
dc.typeThesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (PhD)
dc.publisher.institutionUniversity of Cambridge
dc.publisher.departmentPhysics
dc.date.updated2018-04-08T17:06:20Z
dc.identifier.doi10.17863/CAM.22055
dc.contributor.orcidLuthman, Anna Siri Naemi [0000-0002-8539-826X]
dc.publisher.collegeLucy Cavendish College
dc.type.qualificationtitlePhD in Physics
cam.supervisorBohndiek, Sarah Elizabeth
cam.thesis.fundingfalse
rioxxterms.freetoread.startdate2400-01-01


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