Photoelectrochemistry of Photosystem II in Vitro vs in Vivo.
Journal of the American Chemical Society
American Chemical Society
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Zhang, J., Bombelli, P., Sokol, K., Fantuzzi, A., Rutherford, A. W., Howe, C., & Reisner, E. (2018). Photoelectrochemistry of Photosystem II in Vitro vs in Vivo.. Journal of the American Chemical Society, 140 (1), 6-9. https://doi.org/10.1021/jacs.7b08563
Factors governing the photoelectrochemical output of photosynthetic microorganisms are poorly understood, which can lead to energy losses through inefficient electron transfer (ET) processes. Here, we systematically compare the photoelectrochem-istry of photosystem II (PSII) protein-films to cyanobacteria bio-films to derive: (i) the losses in light-to-charge conversion efficien-cies, (ii) gains in photocatalytic longevity, and (iii) insights into the ET mechanism at the biofilm interface. This study was enabled by the use of hierarchically structured electrodes, which could be tai-lored for high/stable loadings of PSII core complexes and Synecho-cystis sp. PCC 6803 cells. The photocurrent densities generated by the biofilm were two orders of magnitude lower than those of the protein-film. This was attributed to a lower photocatalyst loading since the rate of electron extraction from PSII in vitro is only dou-ble that of PSII in vivo. On the other hand, the biofilm exhibited much greater longevity (>5 days) than the protein-film (<6 h), with turnover numbers surpassing those of the protein-film after 2 days. The mechanism of the electrogenesis was revealed to involve an intracellular redox mediator, which is released during light irradia-tion.
Biofilms, Cyanobacteria, Synechocystis, Photosystem II Protein Complex, Electrodes, Electrochemical Techniques, Photochemical Processes
ECH2020 EUROPEAN RESEARCH COUNCIL (ERC) (682833)
Leverhulme Trust (RPG-2015-393)
Embargo Lift Date
External DOI: https://doi.org/10.1021/jacs.7b08563
This record's URL: https://www.repository.cam.ac.uk/handle/1810/274952
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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