Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana.
Authors
de Vries, Stefan PW
Vurayai, Moses
Gupta, Srishti
Bateman, Michael
Goldfarb, David
Matsheka, Maitshwarelo Ignatius
Publication Date
2018Journal Title
PLoS One
ISSN
1932-6203
Publisher
Public Library of Science (PLoS)
Volume
13
Issue
3
Pages
e0194481
Language
eng
Type
Article
This Version
VoR
Physical Medium
Electronic-eCollection
Metadata
Show full item recordCitation
de Vries, S. P., Vurayai, M., Holmes, M., Gupta, S., Bateman, M., Goldfarb, D., Maskell, D., et al. (2018). Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana.. PLoS One, 13 (3), e0194481. https://doi.org/10.1371/journal.pone.0194481
Abstract
Campylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.
Keywords
Animals, Chickens, Humans, Campylobacter, Campylobacter Infections, Poultry Diseases, Diarrhea, Phylogeny, Drug Resistance, Bacterial, Botswana, Female, Male
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/K004514/1)
Medical Research Council (MR/N002660/1)
Medical Research Council (G1001787)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1371/journal.pone.0194481
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275024
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