Show simple item record

dc.contributor.authorde Vries, Stefan PWen
dc.contributor.authorVurayai, Mosesen
dc.contributor.authorHolmes, Marken
dc.contributor.authorGupta, Srishtien
dc.contributor.authorBateman, Michaelen
dc.contributor.authorGoldfarb, Daviden
dc.contributor.authorMaskell, Duncanen
dc.contributor.authorMatsheka, Maitshwarelo Ignatiusen
dc.contributor.authorGrant, Andrewen
dc.date.accessioned2018-04-19T10:42:55Z
dc.date.available2018-04-19T10:42:55Z
dc.date.issued2018-01en
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/275024
dc.description.abstractCampylobacter spp. are a leading cause of bacterial enteritis worldwide, including countries in Africa, and have been identified by the World Health Organisation (WHO) as one of the high priority antimicrobial resistant pathogens. However, at present there is little knowledge on the prevalence, molecular epidemiology or antimicrobial susceptibility of Campylobacter spp. isolates in Botswana, both in patients and in the zoonotic context. Some data indicate that ~14% of diarrhoeal disease cases in a paediatric setting can be ascribed to Campylobacter spp., urging the need for the magnitude of Campylobacter-associated diarrhoea to be established. In this survey, we have characterised the genomic diversity of Campylobacter spp. circulating in Botswana isolated from cases of diarrhoeal disease in humans (n = 20) and from those that colonised commercial broiler (n = 35) and free-range (n = 35) chickens. Phylogeny showed that the Campylobacter spp. isolated from the different poultry and human sources were highly related, suggesting that zoonotic transmission has likely occurred. We found that for Campylobacter spp. isolated from humans, broilers and free-range chickens, 52% was positive for tetO, 47% for gyrA-T86I, 72% for blaOXA-61, with 27% carrying all three resistance determinants. No 23S mutations conferring macrolide resistance were detected in this survey. In summary, our study provides insight into Campylobacter spp. in poultry reservoirs and in diarrhoeal patients, and the relevance for treatment regimens in Botswana.
dc.format.mediumElectronic-eCollectionen
dc.languageengen
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAnimalsen
dc.subjectChickensen
dc.subjectHumansen
dc.subjectCampylobacteren
dc.subjectCampylobacter Infectionsen
dc.subjectPoultry Diseasesen
dc.subjectDiarrheaen
dc.subjectPhylogenyen
dc.subjectDrug Resistance, Bacterialen
dc.subjectBotswanaen
dc.subjectFemaleen
dc.subjectMaleen
dc.titlePhylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana.en
dc.typeArticle
prism.issueIdentifier3en
prism.publicationDate2018en
prism.publicationNamePloS oneen
prism.startingPagee0194481
prism.volume13en
dc.identifier.doi10.17863/CAM.22186
dcterms.dateAccepted2018-03-05en
rioxxterms.versionofrecord10.1371/journal.pone.0194481en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-01en
dc.contributor.orcidHolmes, Mark [0000-0002-5454-1625]
dc.contributor.orcidGoldfarb, David [0000-0003-0835-9504]
dc.contributor.orcidMaskell, Duncan [0000-0002-5065-653X]
dc.contributor.orcidGrant, Andrew [0000-0001-9746-2989]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/K004514/1)
pubs.funder-project-idMEDICAL RESEARCH COUNCIL (MR/N002660/1)
pubs.funder-project-idMRC (G1001787)
cam.orpheus.successThu Jan 30 13:00:36 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


Files in this item

Thumbnail
Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International