Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis.
Authors
Strauss, Bernhard
Publication Date
2018-01Journal Title
The Journal of cell biology
ISSN
0021-9525
Publisher
Rockefeller University Press
Volume
217
Issue
1
Pages
179-193
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print-Electronic
Metadata
Show full item recordCitation
Strauss, B., Harrison, A., Coelho, P. A., Yata, K., Zernicka-Goetz, M., & Pines, J. (2018). Cyclin B1 is essential for mitosis in mouse embryos, and its nuclear export sets the time for mitosis.. The Journal of cell biology, 217 (1), 179-193. https://doi.org/10.1083/jcb.201612147
Abstract
There is remarkable redundancy between the Cyclin-Cdk complexes that comprise the
cell cycle machinery: none of the mammalian A, D or E-type cyclins is required in
development until implantation and only Cdk1 is essential for early cell divisions.
Cyclin B1 is essential for development but whether it is required for cell division is
contentious. Here, we used a novel imaging approach to analyze Cyclin B1 null
embryos from fertilisation onwards. We show that Cyclin B1-/- embryos arrest in G2
phase after just two divisions. This is the earliest arrest of any Cyclin known and
places Cyclin B1 with cdk1 as the essential regulators of the cell cycle. We
reintroduced mutant proteins into this genetically null background to ask why Cyclin
B1 is constantly exported from the nucleus. We find that Cyclin B1 must be exported
from the nucleus for the cell to prevent premature entry to mitosis, and retaining
Cyclin B1-Cdk1 at the plasma membrane precludes entry to mitosis.
Keywords
Animals, Mice, Knockout, Mice, CDC2 Protein Kinase, Cell Cycle Proteins, DNA-Binding Proteins, Nuclear Proteins, Transcription Factors, Mitosis, Active Transport, Cell Nucleus, Phosphorylation, Embryonic Development, Protein-Tyrosine Kinases, cdc25 Phosphatases, Cyclin B1
Sponsorship
Wellcome Trust (092096/Z/10/Z)
Cancer Research UK (A14492)
Isaac Newton Trust (MINUTE 726(K))
MRC (G1000818)
MRC (G0701184)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1083/jcb.201612147
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275414
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