Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.
Authors
Shastry, Rajesh Padumane
Vittal, Ravishankar Rai
Publication Date
2018-05Journal Title
FEMS microbiology letters
ISSN
0378-1097
Publisher
Oxford University Press
Volume
365
Issue
9
Language
eng
Type
Article
This Version
VoR
Physical Medium
Print
Metadata
Show full item recordCitation
Shastry, R. P., Dolan, S., Abdelhamid, Y., Vittal, R. R., & Welch, M. (2018). Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.. FEMS microbiology letters, 365 (9)https://doi.org/10.1093/femsle/fny054
Abstract
The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from CS66 that was 96% identical to the well-characterised AHL-lactonase AiiA from Bacillus thuringiensis, but only 30% identical to AHL-lactonases from other Gram-negative species. This confirms that close AiiA homologs can be found in both Gram-positive and Gram-negative bacteria. Purified AiiE exhibited potent AHL-lactonase activity against a broad range of AHLs. Furthermore, aiiE was able to reduce the production of secreted plant cell wall-degrading hydrolytic enzymes when expressed in trans in the economically-important plant pathogen, Pectobacterium atrosepticum. Our results indicate the presence of a novel AHL-lactonase in Enterobacter sp. CS66 with significant potential as a biocontrol agent.
Keywords
Enterobacter, Ligases, Bacterial Proteins, Sequence Alignment, Amino Acid Sequence, Kinetics, Quorum Sensing, Acyl-Butyrolactones, Endophytes, Magnoliopsida
Sponsorship
The authors are thankful to Department of Science and Technology, New Delhi, for providing a fellowship for RPS (Grant order number DST/INSPIRE Fellowship/2011(265), 17th July 2011) and for the award of Newton Bhabha PhD Placement Programme-2016 supporting RPS during his secondment to the laboratory of MW. YA is supported by the Yousef Jameel Foundation. SKD is supported by the BBSRC. Work on quorum sensing in the MW laboratory is supported by the BBSRC and the Rosetrees Trust.
Funder references
BBSRC (BB/M019411/1)
Rosetrees Trust (A1318)
Embargo Lift Date
2100-01-01
Identifiers
External DOI: https://doi.org/10.1093/femsle/fny054
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275871
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