Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.
Shastry, Rajesh Padumane
Vittal, Ravishankar Rai
FEMS microbiology letters
Oxford University Press
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Shastry, R. P., Dolan, S., Abdelhamid, Y., Vittal, R. R., & Welch, M. (2018). Purification and characterisation of a quorum quenching AHL-lactonase from the endophytic bacterium Enterobacter sp. CS66.. FEMS microbiology letters, 365 (9)https://doi.org/10.1093/femsle/fny054
The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from CS66 that was 96% identical to the well-characterised AHL-lactonase AiiA from Bacillus thuringiensis, but only 30% identical to AHL-lactonases from other Gram-negative species. This confirms that close AiiA homologs can be found in both Gram-positive and Gram-negative bacteria. Purified AiiE exhibited potent AHL-lactonase activity against a broad range of AHLs. Furthermore, aiiE was able to reduce the production of secreted plant cell wall-degrading hydrolytic enzymes when expressed in trans in the economically-important plant pathogen, Pectobacterium atrosepticum. Our results indicate the presence of a novel AHL-lactonase in Enterobacter sp. CS66 with significant potential as a biocontrol agent.
Enterobacter, Ligases, Bacterial Proteins, Sequence Alignment, Amino Acid Sequence, Kinetics, Quorum Sensing, Acyl-Butyrolactones, Endophytes, Magnoliopsida
The authors are thankful to Department of Science and Technology, New Delhi, for providing a fellowship for RPS (Grant order number DST/INSPIRE Fellowship/2011(265), 17th July 2011) and for the award of Newton Bhabha PhD Placement Programme-2016 supporting RPS during his secondment to the laboratory of MW. YA is supported by the Yousef Jameel Foundation. SKD is supported by the BBSRC. Work on quorum sensing in the MW laboratory is supported by the BBSRC and the Rosetrees Trust.
Rosetrees Trust (A1318)
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External DOI: https://doi.org/10.1093/femsle/fny054
This record's URL: https://www.repository.cam.ac.uk/handle/1810/275871
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/
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