Peroxisomal 2-Hydroxyacyl-CoA Lyase Is Involved in Endogenous Biosynthesis of Heptadecanoic Acid.
View / Open Files
Publication Date
2017-10-13Journal Title
Molecules
ISSN
1420-3049
Publisher
MDPI AG
Volume
22
Issue
10
Language
eng
Type
Article
This Version
VoR
Physical Medium
Electronic
Metadata
Show full item recordCitation
Jenkins, B., de Schryver, E., Van Veldhoven, P. P., & Koulman, A. (2017). Peroxisomal 2-Hydroxyacyl-CoA Lyase Is Involved in Endogenous Biosynthesis of Heptadecanoic Acid.. Molecules, 22 (10) https://doi.org/10.3390/molecules22101718
Abstract
Circulating heptadecanoic acid (C17:0) is reported to be a pathology risk/prognosis biomarker and a dietary biomarker. This pathology relationship has been shown to be reliably predictive even when independent of dietary contributions, suggesting that the endogenous biosynthesis of C17:0 is related to the pathological aetiology. Little is known about C17:0 biosynthesis, which tissues contribute to the circulating levels, and how C17:0 is related to pathology. Hacl1+/- mice were mated to obtain Hacl1-/- and Hacl1+/+ control mice. At 14 weeks, they were anesthetized for tissue collection and fatty acid analysis. Compared to Hacl1+/+, C15:0 was not significantly affected in any Hacl1-/- tissues. However, the Hacl1-/- plasma and liver C17:0 levels were significantly lower: ~26% and ~22%, respectively. No significant differences were seen in the different adipose tissues. To conclude, Hacl1 plays a significant role in the liver and plasma levels of C17:0, providing evidence it can be endogenously biosynthesized via alpha-oxidation. The strong inverse association of C17:0 with pathology raises the question whether there is a direct link between α-oxidation and these diseases. Currently, there is no clear evidence, warranting further research into the role of α-oxidation in relation to metabolic diseases.
Keywords
Liver, Adipose Tissue, Peroxisomes, Animals, Mice, Knockout, Humans, Mice, Carbon-Carbon Lyases, Enoyl-CoA Hydratase, Fatty Acids, Oxidation-Reduction, Tissue Distribution
Sponsorship
National Institute for Health Research (NIHR) (unknown)
Biotechnology and Biological Sciences Research Council (BB/M027252/2)
MRC (TT/5107)
Biotechnology and Biological Sciences Research Council (BB/P028195/1)
Biotechnology and Biological Sciences Research Council (BB/M027252/1)
Identifiers
External DOI: https://doi.org/10.3390/molecules22101718
This record's URL: https://www.repository.cam.ac.uk/handle/1810/277082
Statistics
Total file downloads (since January 2020). For more information on metrics see the
IRUS guide.
Recommended or similar items
The following licence files are associated with this item: