Hepatic steatosis risk is partly driven by increased de novo lipogenesis following carbohydrate consumption.
Authors
Sanders, Francis WB
Acharjee, Animesh
Walker, Celia
Marney, Luke
Roberts, Lee D
Jenkins, Benjamin
Case, Jack
Ray, Sumantra
Virtue, Samuel
Kuh, Diana
Hardy, Rebecca
Allison, Michael
Murray, Andrew J
Wareham, Nick
Griffin, Julian L
Publication Date
2018-06-20Journal Title
Genome Biol
ISSN
1474-7596
Publisher
Springer Science and Business Media LLC
Type
Journal Article
Metadata
Show full item recordCitation
Sanders, F. W., Acharjee, A., Walker, C., Marney, L., Roberts, L. D., Imamura, F., Jenkins, B., et al. (2018). Hepatic steatosis risk is partly driven by increased de novo lipogenesis following carbohydrate consumption.. [Journal Article]. https://doi.org/10.1186/s13059-018-1439-8
Abstract
BACKGROUND: Diet is a major contributor to metabolic disease risk, but there is controversy as to whether increased incidences of diseases such as non-alcoholic fatty liver disease arise from consumption of saturated fats or free sugars. Here, we investigate whether a sub-set of triacylglycerols (TAGs) were associated with hepatic steatosis and whether they arise from de novo lipogenesis (DNL) from the consumption of carbohydrates. RESULTS: We conduct direct infusion mass spectrometry of lipids in plasma to study the association between specific TAGs and hepatic steatosis assessed by ultrasound and fatty liver index in volunteers from the UK-based Fenland Study and evaluate clustering of TAGs in the National Survey of Health and Development UK cohort. We find that TAGs containing saturated and monounsaturated fatty acids with 16-18 carbons are specifically associated with hepatic steatosis. These TAGs are additionally associated with higher consumption of carbohydrate and saturated fat, hepatic steatosis, and variations in the gene for protein phosphatase 1, regulatory subunit 3b (PPP1R3B), which in part regulates glycogen synthesis. DNL is measured in hyperphagic ob/ob mice, mice on a western diet (high in fat and free sugar) and in healthy humans using stable isotope techniques following high carbohydrate meals, demonstrating the rate of DNL correlates with increased synthesis of this cluster of TAGs. Furthermore, these TAGs are increased in plasma from patients with biopsy-confirmed steatosis. CONCLUSION: A subset of TAGs is associated with hepatic steatosis, even when correcting for common confounding factors. We suggest that hepatic steatosis risk in western populations is in part driven by increased DNL following carbohydrate rich meals in addition to the consumption of saturated fat.
Keywords
De novo lipogenesis, Direct infusion mass spectrometry, Non-alcoholic fatty liver disease, Triacylglycerols, Triglycerides, Animals, Carbohydrates, Diet, Fatty Liver, Female, Humans, Lipids, Lipogenesis, Liver, Male, Mice, Mice, Inbred C57BL, Mice, Obese, Middle Aged, Risk, Triglycerides
Sponsorship
Medical Research Council (MR/P01836X/1)
Medical Research Council (MC_UU_12015/5)
Department of Health (via National Institute for Health Research (NIHR)) (NF-SI-0617-10149)
Department of Health (via National Institute for Health Research (NIHR)) (NF-SI-0512-10135)
Medical Research Council (MC_UU_12015/1)
MRC (MC_PC_13046)
MRC (Unknown)
MRC (unknown)
Medical Research Council (G0802051)
Medical Research Council (MC_UU_12012/5)
Medical Research Council (MC_UU_12012/2)
Medical Research Council (MR/P011705/1)
Medical Research Council (MC_EX_MR/L100002/1)
Medical Research Council (MC_PC_13030)
British Heart Foundation (None)
Medical Research Council (G0600717)
Medical Research Council (MC_G0802535)
Medical Research Council (G0400192)
Fondazione Umberto Veronesi (unknown)
MRC (MC_UU_00014/2)
MRC (MC_UU_00014/5)
Identifiers
External DOI: https://doi.org/10.1186/s13059-018-1439-8
This record's DOI: https://doi.org/10.17863/CAM.24589
Rights
Rights Holder: The Author(s).
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