Telomerase mediates lymphocyte proliferation but not the atherosclerosis-suppressive potential of regulatory T-cells
Al Zhrany, N
Arteriosclerosis, Thrombosis, and Vascular Biology
Wolters Kluwer Health
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Richardson, G., Sage, A., Bennaceur, K., Al Zhrany, N., Coelho-Lima, J., Dookun, E., Draganova, L., et al. (2018). Telomerase mediates lymphocyte proliferation but not the atherosclerosis-suppressive potential of regulatory T-cells. Arteriosclerosis, Thrombosis, and Vascular Biology https://doi.org/10.1161/ATVBAHA.117.309940
Objective: Atherosclerosis is an age-related disease characterised by systemic oxidative stress and low-grade inflammation. The role of telomerase and telomere length in atherogenesis remains contentious. Short telomeres of peripheral leukocytes are predictive for coronary artery disease. Conversely, attenuated telomerase has been demonstrated to be protective for atherosclerosis. Hence a potential causative role of telomerase in atherogenesis is critically debated. Approach and Results: In this study we used multiple mouse models to investigate the regulation of telomerase under oxidative stress as well as its impact on atherogenesis in vitro and in vivo. Using primary lymphocytes and myeloid cell cultures we demonstrate that cultivation under hyperoxic conditions induced oxidative stress resulting in chronic activation of CD4+ cells and significantly reduced CD4+ T-cell proliferation. The latter was telomerase dependent, as oxidative stress had no effect on the proliferation of primary lymphocytes isolated from telomerase-knock-out mice. In contrast, myeloid cell proliferation was unaffected by oxidative stress nor reliant on telomerase. Telomerase reverse transcriptase (TERT) deficiency had no effect on Treg numbers in vivo or suppressive function ex vivo. Adoptive transfer of TERT-/- Tregs into Rag2-/- ApoE-/- double knock out mice demonstrated that telomerase function was not required for the ability of Tregs to protect against atherosclerosis. However, telomere length was critical for Treg function. Conclusions: Telomerase contributes to lymphocyte proliferation but plays no major role in Treg function, provided that telomere length is not critically short. We suggest that oxidative stress may contribute to atherosclerosis via suppression of telomerase and acceleration of telomere attrition in Tregs.
atherosclerosis, lymphocytes, models, animal, oxidative stress, telomerase
This study was supported, in part, by British Heart Foundation Project Grants PG/15/85/31744 and PG/12/47/29681 (www.BHF.org.uk) as well as the Newcastle Healthcare Charity (www.newcastle-hospitals. org.uk/patient-guides/charity-matters-at-newcastle-hospitals_charitable- funds.aspx). N.M. Al Zhrany was funded by a stipend from the Government of Saudi Arabia.
British Heart Foundation (CH/10/001/27642)
British Heart Foundation (RG/15/11/31593)
External DOI: https://doi.org/10.1161/ATVBAHA.117.309940
This record's URL: https://www.repository.cam.ac.uk/handle/1810/277798
Attribution 4.0 International
Licence URL: http://creativecommons.org/licenses/by/4.0/