Transcription factors PRE3 and WOX11 are involved in the formation of new lateral roots from secondary growth taproot in A. thaliana.
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Baesso, B., Chiatante, D., Terzaghi, M., Zenga, D., Nieminen, K., Mahonen, A., Siligato, R., et al. (2018). Transcription factors PRE3 and WOX11 are involved in the formation of new lateral roots from secondary growth taproot in A. thaliana.. Plant Biology, 20 (3), 426-432. https://doi.org/10.1111/plb.12711
The spatial deployment of lateral roots determines the ability of a plant to interact with the surrounding environment for nutrition and anchorage. This paper shows that besides the pericycle, the vascular cambium becomes active in Arabidopsis thaliana taproot at a later stage of development and is also able to form new lateral roots. To demonstrate the above, we implemented a two-step approach in which the first step leads to development of a secondary structure in A. thaliana taproot, and the second applies a mechanical stress on the vascular cambium to initiate formation of a new lateral root primordium. GUS staining showed PRE3, DR5 and WOX11 signals in the cambial zone of the root during new lateral root formation. An advanced level of wood formation, characterized by the presence of medullar rays, was achieved. Preliminary investigations suggest the involvement of auxin and two transcription factors (PRE3/ATBS1/bHLH135/TMO7 and WOX11) in the transition of some vascular cambium initials from a role as producers of xylem/phloem mother cells to founder cells of a new lateral root primordium.
auxin, bending, lateral root, PRE3, secondary structure, WOX11
University of Insubria (FAR) EC FP7 Project. Grant Number: ZEPHYR‐308313 Gatsby Foundation. Grant Number: GAT3395/PR3 National Science Foundation Biotechnology and Biological Sciences Research Council. Grant Number: BB/N013158/1 University of Helsinki. Grant Number: 799992091 European Research Council. Grant Number: 323052 Academy of Finland Centre of Excellence programme. Grant Number: 63053034
European Research Council (323052)
Gatsby Charitable Foundation (GAT3395/PR3)
External DOI: https://doi.org/10.1111/plb.12711
This record's URL: https://www.repository.cam.ac.uk/handle/1810/278493